Ma Xiaomeng, Guan Long, Xuan Junli, Wang Huihua, Yuan Zehu, Wu Mingming, Liu Ruizao, Zhu Caiye, Wei Caihong, Zhao Fuping, Du Lixin, Zhang Li
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Anim Genet. 2016 Oct;47(5):618-22. doi: 10.1111/age.12455. Epub 2016 Jul 20.
Our previous genome-wide association study in sheep revealed that OAR3-84073899.1 (SNP31) in intron 8 of the CAMKMT gene was significantly associated with post-weaning gain at the genomic level. Herein, we performed a replication study to investigate single nucleotide polymorphisms (SNPs) within the CAMKMT gene exons, and 1000 bp of the 5'- and 3'-intranslated regions (UTRs) and their associations with growth traits in Ujumqin sheep. Five SNPs were identified through DNA pool sequencing technology: SNP26 in the 5'-UTR, SNP06 in exon 5, SNP07 in exon 8 and SNP27 and SNP28 in the 3'-UTR. Six SNPs, including SNP31 in intron 8, were genotyped in the validation group of 343 Ujumqin sheep, and each SNP was classified into three genotypes. The chi-square test suggested that all the variations were in Hardy-Weinberg equilibrium (P > 0.05) except for SNP28 and SNP31. Linkage disequilibrium analysis showed that SNP07 and SNP31 were strongly linked. An association analysis suggested that SNP06 was significantly associated with chest girth at 6 months of age (P < 0.05). SNP07 exhibited significant correlation with body weight and chest girth at 4 months of age and with body weight, chest girth and chest width at 6 months of age (P < 0.05). SNP27 was highly associated with body weight and chest girth at 4 months of age (P < 0.05), and SNP28 was extremely significantly associated with body weight and chest girth at 4 months of age and with chest girth at 6 months of age (P < 0.01). SNP31 was significantly associated with body weight and shin circumference at 4 months of age and with post-weaning gain (P < 0.05). Association analysis of the combined effect of SNP07 and SNP31 showed significant correlation with body weight and chest girth at four of months of age (P < 0.05) and with body weight and chest girth at 6 months of age (P < 0.05). These results indicate that the SNPs could be used as meritorious and available genetic markers in growth traits breeding and that the CAMKMT gene may be one of the key candidate genes that affect Ujumqin economic traits.
我们之前对绵羊进行的全基因组关联研究表明，钙调蛋白激酶甲基转移酶（CAMKMT）基因第8内含子中的OAR3-84073899.1（SNP31）在基因组水平上与断奶后增重显著相关。在此，我们进行了一项重复研究，以调查CAMKMT基因外显子内的单核苷酸多态性（SNP），以及5'和3'非翻译区（UTR）的1000 bp及其与乌珠穆沁羊生长性状的关联。通过DNA池测序技术鉴定出5个SNP：5'-UTR中的SNP26、外显子5中的SNP06、外显子8中的SNP07以及3'-UTR中的SNP27和SNP28。在343只乌珠穆沁羊的验证组中对包括第8内含子中的SNP31在内的6个SNP进行基因分型，每个SNP分为三种基因型。卡方检验表明，除SNP28和SNP31外，所有变异均处于哈迪-温伯格平衡（P>0.05）。连锁不平衡分析表明，SNP07和SNP31紧密连锁。关联分析表明，SNP06与6月龄胸围显著相关（P<0.05）。SNP07与4月龄体重和胸围以及6月龄体重、胸围和胸宽显著相关（P<0.05）。SNP27与4月龄体重和胸围高度相关（P<0.05），SNP28与4月龄体重和胸围以及6月龄胸围极显著相关（P<0.01）。SNP31与4月龄体重和胫围以及断奶后增重显著相关（P<0.05）。SNP07和SNP31联合效应关联分析表明，与4月龄体重和胸围以及6月龄体重和胸围显著相关（P<0.05）。这些结果表明，这些SNP可作为生长性状育种中有价值且可用的遗传标记，并且CAMKMT基因可能是影响乌珠穆沁羊经济性状的关键候选基因之一。
