Qi Feifei, Qian Liangliang, Liu Jingjing, Li Xiaoqing, Lu Lingeng, Xu Qian
Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
Department of Chronic Disease Epidemiology, Yale School of Public Health, School of Medicine, Yale University, 60 College Street, New Haven, CT 06520-8034, USA.
J Chromatogr A. 2016 Aug 19;1460:24-32. doi: 10.1016/j.chroma.2016.07.020. Epub 2016 Jul 9.
This study used nanofibers mat (NFsM)-based micro-SPE (μ-SPE) in 96-well plate format as a novel high-throughput sample preparation technique prior to the determination of cationic dyes in wastewater using HPLC-DAD. P-Toluene sulfonate (PTS(-)) doped polypyrrole (PPy) functionalized NFsM (PTS-PPy NFsM) was optimized as SPE sorbent in aspects of PPy form (particles and NFsM) and its doped anions (Cl(-) and PTS(-)), which showed good extraction efficiency and adsorption capacity for cationic dyes (Auramine-O, Chrysoidine and Rhodamine-B). Under the optimal conditions, the limits of detection (LODs) were between 0.3 and 0.5μg/L, and the linearity was achieved in the concentration ranging from 1.0 to 150.0μg/L with correlation coefficients (R) between 0.992 and 0.999. Compared with the published SPE methods, this approach demonstrated its advantages such as shorter extraction time (0.3min per sample), lower requirement sorbent (2.0mg), lower volume of organic solvent (0.7mL), higher recovery (90.1-99.1%) and precision (RSDs<6.9%). With this developed method, we have successfully analyzed the dyeing industry wastewater, which meets the Discharge Standards of Water Pollutants for Dyeing and Finishing of Textile Industry in China. The concentrations of three analyzed cationic dyes were from 2.9 to 13.9μg/L. The NFsM-based μ-SPE technique is practically a high-throughput sample preparation procedure that can accurately assess the pollutants in the wastewater from dyeing industry.
本研究采用基于纳米纤维垫（NFsM）的96孔板格式微固相萃取（μ-SPE）作为一种新型高通量样品前处理技术，用于在使用高效液相色谱-二极管阵列检测器（HPLC-DAD）测定废水中阳离子染料之前的样品前处理。对对甲苯磺酸盐（PTS(-)）掺杂的聚吡咯（PPy）功能化NFsM（PTS-PPy NFsM）在PPy形式（颗粒和NFsM）及其掺杂阴离子（Cl(-)和PTS(-)）方面进行了优化，作为固相萃取吸附剂，其对阳离子染料（金胺-O、碱性菊橙和罗丹明-B）显示出良好的萃取效率和吸附容量。在最佳条件下，检测限（LOD）在0.3至0.5μg/L之间，在1.0至150.0μg/L的浓度范围内实现了线性，相关系数（R）在0.992至0.999之间。与已发表的固相萃取方法相比，该方法显示出其优势，如萃取时间较短（每个样品0.3分钟）、吸附剂需求量较低（2.0mg）、有机溶剂用量较少（0.7mL）、回收率较高（90.1-99.1%）和精密度较高（相对标准偏差<6.9%）。采用这种开发的方法，我们成功地分析了印染行业废水，其符合中国纺织染整工业水污染物排放标准。三种分析的阳离子染料浓度为2.9至13.9μg/L。基于NFsM的μ-SPE技术实际上是一种高通量样品前处理程序，能够准确评估印染行业废水中的污染物。
