Department of Environmental Health, Food Safety Division, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Saffron Institute University of Torbat Heydarieh, Torbat Heydarieh, Iran.
Sci Rep. 2024 Apr 8;14(1):8217. doi: 10.1038/s41598-024-58825-x.
The present study of a novel metal-organic framework containing Fe single atoms doped on electrospun carbon nanofibers (Fe SA-MOF@CNF) based on dispersive micro solid phase extraction (D-μ-SPE) using HPLC-PDA for detection tartrazine in fake saffron samples was designed. The Fe SA-MOF@CNF sorbent was extensively characterized through various techniques including N adsorption-desorption isotherms, X-ray diffraction (XRD), scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy. The specific area of surface of the sorbent was 577.384 m/g. The study variables were optimized via the central composite design (CCD), which included a sorbent mass of 15 mg, a contact time of 6 min, a pH of 7.56, and a tartrazine concentration of 300 ng/ml. Under the optimum condition, the calibration curve of this method was linear in the range of 5-1000 ng/mL, with a correlation coefficient of 0.992. The LOD and LOQ values were ranged 0.38-0.74 and 1.34-2.42 ng/ml, respectively. This approach revealed significant improvements, including high extraction recovery (98.64), recovery rates (98.43-102.72%), and accuracy (RSDs < 0.75 to 3.6%). the enrichment factors were obtained in the range of 80.6-86.4 with preconcentration factor of 22.3. Consequently, the D-μ-SPE method based on synthesized Fe SA-MOF@CNF could be recommended as a sustainable sorbent for detecting tartrazine in saffron samples.
本研究设计了一种基于高效液相色谱-光电二极管阵列检测（HPLC-PDA）的新型含 Fe 单原子掺杂于电纺碳纤维上的金属-有机骨架（Fe SA-MOF@CNF）分散微固相萃取（D-μ-SPE），用于检测假冒藏红花样品中的食用柠檬黄。通过 N2 吸附-脱附等温线、X 射线衍射（XRD）、扫描电子显微镜（SEM）和傅里叶变换红外（FTIR）光谱等多种技术对 Fe SA-MOF@CNF 吸附剂进行了广泛的表征。吸附剂的比表面积为 577.384 m2/g。通过中心复合设计（CCD）对研究变量进行了优化，包括吸附剂质量 15mg、接触时间 6min、pH 值 7.56 和食用柠檬黄浓度 300ng/ml。在最佳条件下，该方法的校准曲线在 5-1000ng/ml 范围内呈线性，相关系数为 0.992。LOD 和 LOQ 值分别为 0.38-0.74 和 1.34-2.42ng/ml。该方法显示出显著的改进，包括高萃取回收率（98.64%）、回收率（98.43%-102.72%）和准确度（RSDs<0.75-3.6%）。富集因子在 80.6-86.4 范围内，浓缩因子为 22.3。因此，基于合成的 Fe SA-MOF@CNF 的 D-μ-SPE 方法可以推荐作为一种可持续的藏红花样品中食用柠檬黄的检测方法。
