Laboratory of Environmental Biology, Department of Applied Aquabiology, National Fisheries University, Yamaguchi, Japan.
Yamaguchi Prefectural Fisheries Research Center, Yamaguchi, Japan.
Sci Rep. 2016 Jul 20;6:29923. doi: 10.1038/srep29923.
The aim of this study is to demonstrate the growth-promoting effect of alginate hydrolysates (AHs) on the Manila clam Ruditapes philippinarum, and to verify the physiological change occurring within a living R. philippinarum stimulated by AHs. We show that growth of clams was dramatically promoted by supplementing a diet of the diatom Chaetoceros neogracile with AHs at 4 mg/L. Furthermore, metabolomics indicates that each state of starvation, food satiation, and sexual maturation have a characteristic pattern. In the groups given AHs in addition to C. neogracile in particular, excess carbohydrate was actively utilized for the development of reproductive tissue. In contrast, it appeared that clams in the groups given C. neogracile only were actively growing, utilizing their adequate carbohydrate resources. Meanwhile, the unfed groups have slowed growth because of the lack of an energy source. Hence, supplementation of AHs in addition to the algal diet may be an inexpensive way to shorten the rearing period of R. philippinarum. Moreover, metabolomics can evaluate the growth condition of R. philippinarum in a comprehensive way, and this approach is crucially important for not only the development of a mass culture method but also for the conservation of the clam resource in the field.
本研究旨在展示藻酸盐水解物（AHs）对菲律宾蛤仔（Ruditapes philippinarum）的促生长作用，并验证受 AHs 刺激的活体菲律宾蛤仔体内发生的生理变化。研究表明，在以硅藻新月菱形藻（Chaetoceros neogracile）为食的饮食中添加 4mg/L 的 AHs，可显著促进蛤仔生长。此外，代谢组学表明，饥饿、饱食和性成熟的每个状态都有其特征模式。在添加 AHs 的各组中，特别是在添加 AHs 和新月菱形藻的组中，多余的碳水化合物被积极用于生殖组织的发育。相比之下，只给予新月菱形藻的组中的蛤仔似乎在积极生长，利用其充足的碳水化合物资源。同时，未投喂组由于缺乏能量来源而生长缓慢。因此，在藻类饮食中添加 AHs 可能是缩短菲律宾蛤仔养殖期的一种廉价方法。此外，代谢组学可以全面评估菲律宾蛤仔的生长状况，这种方法不仅对大规模养殖方法的发展至关重要，而且对现场蛤仔资源的保护也至关重要。
