Sundberg-Kövamees Marianne, Grunewald Johan, Wahlström Jan
Respiratory Medicine Unit, Department of Medicine Solna and Center for Molecular Medicine, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden; Lung Allergy Clinic, Karolinska University Hospital, Stockholm, Sweden.
Respiratory Medicine Unit, Department of Medicine Solna and Center for Molecular Medicine, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden; Lung Allergy Clinic, Karolinska University Hospital, Stockholm, Sweden.
Int J Infect Dis. 2016 Nov;52:1-8. doi: 10.1016/j.ijid.2016.07.004. Epub 2016 Jul 18.
Streptococcus pneumonia is a major cause of morbidity and mortality in children and adults worldwide. Lack of fully effective pneumococcal vaccines is a problem. Streptococcus pneumoniae exposes on its surface C-polysaccharide (cell wall polysaccharide, CWPS) and serospecific capsular polysaccharides, used in pneumococcal vaccines. We investigated the effect of CWPS and individual capsular polysaccharides, with regard to activation of subsets of immune cells of healthy controls. Three different capsular polysaccharides, CWPS and LPS were used for in vitro stimulation of whole blood. Cell activation (CD69 expression) was assessed in CD4+ and CD4- T cells, NK-like T cells, NK cells and monocytes by flow cytometry. Cytokine levels in supernatants were quantified by Cytometric Bead Array (CBA). CWPS and the capsules activated immune cell subsets, but to different degrees. NK cells and NK-like T cells showed the strongest activation, followed by monocytes. Among the three capsules, capsule type 23 induced the strongest activation and cytokine release, followed by type 9 and type 3. This study increases the understanding of how the human immune system reacts to pneumococcal vaccine components.
肺炎链球菌是全球儿童和成人发病和死亡的主要原因。缺乏完全有效的肺炎球菌疫苗是一个问题。肺炎链球菌在其表面暴露C-多糖（细胞壁多糖，CWPS）和血清特异性荚膜多糖，这些被用于肺炎球菌疫苗。我们研究了CWPS和单个荚膜多糖对健康对照免疫细胞亚群激活的影响。使用三种不同的荚膜多糖、CWPS和脂多糖对全血进行体外刺激。通过流式细胞术评估CD4+和CD4-T细胞、NK样T细胞、NK细胞和单核细胞中的细胞激活（CD69表达）。通过细胞因子珠阵列（CBA）对上清液中的细胞因子水平进行定量。CWPS和荚膜激活了免疫细胞亚群，但程度不同。NK细胞和NK样T细胞显示出最强的激活，其次是单核细胞。在三种荚膜中，23型荚膜诱导的激活和细胞因子释放最强，其次是9型和3型。这项研究增加了对人类免疫系统如何对肺炎球菌疫苗成分作出反应的理解。
