Robic Annie, Feve Katia, Louveau Isabelle, Riquet Juliette, Prunier Armelle
INRA, UMR1388-GenPhySE, Castanet Tolosan, France.
INRA, UMR1348-PEGASE, Saint-Gilles, France.
Anim Sci J. 2016 Aug;87(8):1041-7. doi: 10.1111/asj.12532. Epub 2015 Dec 21.
To explore the metabolism of steroids in the pig species, a qualitative PCR analysis was performed for the main transcript of 27 genes involved in steroid metabolism. We compared samples of testes, adipose tissue and liver from immature and peripubertal males, adrenal cortex from peripubertal males, ovaries from cyclic females and adipose tissue from peripubertal females. Some genes were shown to have a tissue-specific expression. Two of them were expressed only in testes, ovaries and adrenals: CYP11A1 and CYP11B. The CYP21 and HSD17B3 genes, were expressed respectively only in adrenals and only in testes. Very few differences were observed between transcriptional patterns of peripubertal testes and adrenal glands as well as between male and female fat tissues. However, the expression of genes involved in the sulfonation of steroids was higher in testes than in adrenals from males. Main differences between ovaries and testes were observed for HSD17B1/2/3, AKR1C-pig6 and sulfotransferase genes (SULT2A1/SULT2B1). The present study shows that the SRD5A2 and CYP21 genes were not involved in the testicular biosynthesis of androstenone. It also shows that porcine adrenal glands produce essentially corticosteroids and that fat tissue is unable to produce de novo steroids.
为了探究猪体内类固醇的代谢情况，对参与类固醇代谢的27个基因的主要转录本进行了定性PCR分析。我们比较了未成熟和青春期前雄性的睾丸、脂肪组织和肝脏样本，青春期前雄性的肾上腺皮质，周期性发情雌性的卵巢以及青春期前雌性的脂肪组织。结果显示，一些基因具有组织特异性表达。其中两个基因仅在睾丸、卵巢和肾上腺中表达：CYP11A1和CYP11B。CYP21和HSD17B3基因分别仅在肾上腺和睾丸中表达。在青春期前睾丸和肾上腺的转录模式之间以及雄性和雌性脂肪组织之间观察到的差异非常少。然而，参与类固醇磺化的基因在雄性睾丸中的表达高于肾上腺。在HSD17B1/2/3、AKR1C - pig6和磺基转移酶基因（SULT2A1/SULT2B1）方面观察到卵巢和睾丸之间存在主要差异。本研究表明，SRD5A2和CYP21基因不参与雄烯酮的睾丸生物合成。研究还表明，猪肾上腺主要产生皮质类固醇，脂肪组织无法从头合成类固醇。
