Bonczkowski Pawel, De Scheerder Marie-Angélique, De Spiegelaere Ward, Vandekerckhove Linos
HIV Translational Research Unit, Department of Internal Medicine, Faculty of Medicine and Health Sciences, Ghent University and Ghent University Hospital, Ghent, Belgium.
AIDS Rev. 2016 Oct-Dec;18(4):171-183.
Due to the scarcity of HIV-1 latently infected cells in patients, in vitro primary latency models are now commonly used to study the HIV-1 reservoir. To this end, a number of experimental systems have been developed. Most of these models differ based on the nature of the primary CD4+ T-cell type, the used HIV strains, activation methods, and latency assessment strategies. Despite these differences, most models share some common characteristics. Here, we provide a systematic review covering the primary HIV latency models that have been used to date with the aim to compare these models and identify minimal requirements for such experiments. A systematic search on PubMed and Web of Science databases generated a short list of 17 unique publications that propose new in vitro latency models. Based on the described methods, we propose and discuss a generalized workflow, visualizing all the necessary steps to perform such an in vitro study, with the key choices and validation steps that need to be made; from cell type selection until the model readout.
由于患者体内HIV-1潜伏感染细胞稀缺，目前体外原代潜伏模型常用于研究HIV-1储存库。为此，已开发了许多实验系统。这些模型大多因原代CD4+ T细胞类型的性质、所用HIV毒株、激活方法和潜伏评估策略而有所不同。尽管存在这些差异，但大多数模型具有一些共同特征。在此，我们提供了一项系统综述，涵盖了迄今为止使用的原代HIV潜伏模型，旨在比较这些模型并确定此类实验的最低要求。在PubMed和Web of Science数据库上进行的系统检索产生了一份简短清单，其中包含17篇提出新的体外潜伏模型的独特出版物。基于所描述的方法，我们提出并讨论了一个通用工作流程，直观展示了进行此类体外研究所需的所有步骤，以及需要做出的关键选择和验证步骤；从细胞类型选择到模型读数。
