Ionel Anca, Lucaciu Ondine, Tăbăran Flaviu, Berce Cristian, Toader Septimiu, Hurubeanu Lucia, Bondor Cosmina, Câmpian Radu S
Department of Oral Rehabilitation, Oral Health and Dental Office Management, Faculty of Dental Medicine, "Iuliu Haţieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Department of Anatomic Pathology, Necropsy and Forensic Medicine, Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine, Cluj-Napoca, Romania.
Histol Histopathol. 2017 Apr;32(4):379-384. doi: 10.14670/HH-11-803. Epub 2016 Jul 21.
Periodontal disease with its systemic implications is highly prevalent among the population, and this correlation could have an impact on the quality lives of many humans. The purpose of this study was to assess the clinical and histopathological changes of the periodontium correlated with the systemic inflammatory response in periodontal disease. An experimental study was performed on male Wistar rats which were subjected to a procedure of periodontitis induction through placing silk thread ligatures around the lower incisors, under general anesthesia. Clinically, the changes of the periodontal tissue induced by the periodontitis progression were daily assessed. Two blood samples were obtained from each animal, at baseline and on completion of the experiment. The plasma level of the cytokine IL-6 and haematological parameters such as leukocytes, neutrophils, lymphocytes, monocytes, and platelets were determined. After seven days the animals were sacrificed, and samples were prepared for histological evaluation. Clinical manifestations such as changes in the color, contour and consistency of the gingival tissue and the bleeding on probing were registered. Histopathological analysis showed an intense inflammatory cell infiltration, the presence of osteoclasts and an obvious bone resorption activity. A significant increase in IL-6 values during the progression of periodontitis in rats (p<0.001) was also observed. The results of this research demonstrated that the clinical and histological changes in the rat's periodontium are correlated with a notable systemic inflammatory response. Therefore, periodontitis control can be inserted as part of the programs of systemic disorders prevention, in clinical practice.
牙周疾病及其对全身的影响在人群中高度流行，这种关联可能会对许多人的生活质量产生影响。本研究的目的是评估牙周炎中与全身炎症反应相关的牙周组织的临床和组织病理学变化。对雄性Wistar大鼠进行了一项实验研究，在全身麻醉下，通过在下颌切牙周围放置丝线结扎来诱导牙周炎。临床上，每天评估牙周炎进展引起的牙周组织变化。在基线和实验结束时从每只动物采集两份血样。测定细胞因子IL-6的血浆水平以及白细胞、中性粒细胞、淋巴细胞、单核细胞和血小板等血液学参数。七天后处死动物，并制备样本进行组织学评估。记录牙龈组织的颜色、外形和质地变化以及探诊出血等临床表现。组织病理学分析显示有强烈的炎性细胞浸润、破骨细胞的存在以及明显的骨吸收活动。还观察到大鼠牙周炎进展过程中IL-6值显著升高（p<0.001）。本研究结果表明，大鼠牙周组织的临床和组织学变化与明显的全身炎症反应相关。因此，在临床实践中，控制牙周炎可作为预防全身性疾病方案的一部分。
