Giménez-Siurana Ana, Gómez García Francisco, Pagan Bernabeu Ana, Lozano-Pérez Antonio Abel, Aznar-Cervantes Salvador D, Cenis José Luis, López-Jornet Pía
Department of Bucal Medicine, Faculty of Medicine, University of Murcia, Av. Marqués de los Vélez, 2 Floor. 3008, Murcia, Spain.
Department of Biotechnology, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), C/Mayor 30150 La Alberca, Murcia, Spain.
Antioxidants (Basel). 2020 Jan 19;9(1):85. doi: 10.3390/antiox9010085.
the objective of the present work is to study the effectiveness of treatment with silk fibroin nanoparticles loaded with resveratrol in experimental periodontitis in a diabetic rat model.
Periodontitis is an inflammatory pathology highly related to other diseases, such as type II diabetes. Both diseases have a specific inflammatory condition, with Interleukin (IL)-6, IL-1β and Transforming Grow Factor (TGF)-1β being the most relevant proinflammatory factors. Silk fibroin (SF) nanoparticles loaded with resveratrol (Res-SFN) are a new alternative as a treatment.
40 diabetic Sprague Dawley male rats were used and periodontitis was induced by ligation. The animals were divided into 5 treatment groups, and 1 mL of treatment was administered once a day for 4 weeks. The groups were: I: Carboxymethyl cellulose (CMC) 0.8%, II: CMC 0.8% + SF 1%, III: CMC 0.8% + RES-SFN 3 mg/mL, IV: CMC 0.8% + SF 1% + RES-SFN 3 mg/mL, V: Water. A peripheral blood sample was taken every week to quantify the inflammatory profile by ELISA (IL-6, IL-1β and TGF-1β). After 4 weeks the sacrifice was carried out and biopsies of the gum were taken.
Treatment with SF and RES-SFN reduced the amount of chemical inflammation mediators (with the exception of IL-1β in comparisons I-IV and II-IV ( > 0.05)), as well as the anatomopathological variables linked to it, in a significant way ( < 0.05).
treatment with RES-SFN has reduced local inflammation in this experimental periodontitis model.
本研究旨在探讨白藜芦醇负载丝素蛋白纳米粒治疗糖尿病大鼠实验性牙周炎的疗效。
牙周炎是一种与其他疾病高度相关的炎症性疾病，如II型糖尿病。这两种疾病都有特定的炎症状态，白细胞介素（IL）-6、IL-1β和转化生长因子（TGF）-1β是最相关的促炎因子。白藜芦醇负载丝素蛋白（Res-SFN）纳米粒是一种新的治疗选择。
选用40只雄性糖尿病Sprague Dawley大鼠，通过结扎诱导牙周炎。将动物分为5个治疗组，每天给药1 mL，持续4周。分组如下：I：0.8%羧甲基纤维素（CMC）；II：0.8% CMC + 1%丝素蛋白（SF）；III：0.8% CMC + 3 mg/mL RES-SFN；IV：0.8% CMC + 1% SF + 3 mg/mL RES-SFN；V：水。每周采集外周血样本，通过酶联免疫吸附测定（ELISA）定量炎症指标（IL-6、IL-1β和TGF-1β）。4周后处死动物，取牙龈组织活检。
SF和RES-SFN治疗显著降低了化学炎症介质的含量（I-IV组和II-IV组比较中IL-1β除外（> 0.05）），以及与之相关的解剖病理学变量（< 0.05）。
RES-SFN治疗可减轻该实验性牙周炎模型的局部炎症。
