Goglia F, Liverini G, Lanni A, Iossa S, Barletta A
Department of General and Environmental Physiology, University of Naples, Italy.
Mol Cell Endocrinol. 1989 Mar;62(1):41-6. doi: 10.1016/0303-7207(89)90111-1.
In this paper we report that three different rat liver mitochondrial fractions, differing in density, exhibit differential effects when the animals are made hypo- or hyperthyroid. The investigations have been performed by correlating the protein content, the succinic dehydrogenase behaviour and the respiratory features of the three fractions in different thyroid states with morphometric-stereologic analysis the electron micrographic level. The results indicate that the thyroid hormone influences both the mass and the functionality of the heavy (H) and light (L) fraction. In hypothyroid rats the H fraction increases (+43%) while the L fraction decreases (-32%) and their respiratory activity is drastically reduced. Adenosine triphosphate (ATP) synthesis in the H fraction is also inhibited. Triiodothyronine (T3) administration to the above animals restores the values observed in control rats. At morphometric level we note in hypothyroid rats an increase in the number of mitochondria together with a concomitant increase in the average volume of a single mitochondrion. We are inclined to explain the above results through an action exerted by T3 on a hypothetical mitochondrial cycle starting with the formation of light organelles from heavy ones.
在本文中，我们报告称，当动物处于甲状腺功能减退或亢进状态时，三种密度不同的大鼠肝脏线粒体组分表现出不同的效应。通过将不同甲状腺状态下三种组分的蛋白质含量、琥珀酸脱氢酶活性及呼吸特性与电子显微镜水平的形态计量学 - 体视学分析相关联来进行研究。结果表明，甲状腺激素会影响重（H）组分和轻（L）组分的质量及功能。在甲状腺功能减退的大鼠中，H组分增加（+43%），而L组分减少（-32%），并且它们的呼吸活性大幅降低。H组分中的三磷酸腺苷（ATP）合成也受到抑制。对上述动物给予三碘甲状腺原氨酸（T3）可恢复在对照大鼠中观察到的值。在形态计量学水平上，我们注意到甲状腺功能减退的大鼠中线粒体数量增加，同时单个线粒体的平均体积也随之增加。我们倾向于通过T3对一个假设的线粒体循环的作用来解释上述结果，该循环始于从重线粒体形成轻细胞器。
