Jakovcic S, Swift H H, Gross N J, Rabinowitz M
J Cell Biol. 1978 Jun;77(3):887-901. doi: 10.1083/jcb.77.3.887.
The concentrations of the inner mitochondrial membrane markers cardiolipin and cytochrome alpha have been measured in liver homogenates and in purified mitochondria after thyroxine administration to thyroidectomized and normal rats. The biochemical results have been correlated with stereological electron micrographic analyses of hepatocytes in liver sections, and of isolated mitochondrial pellets. There were progressive and parallel increases in homogenate and mitochondrial cardiolipin concentration, and in mitochondrial cytochrome alpha concentration, after administration of 20 microgram of thyroxine on alternate days to thyroidectomized rats, and of 300 microgram on alternate days to normal rats. Electron microscope measurements showed marked differences in the shape of the mitochondria and in the number of cristae in different thyroid states. Hypothyroid mitochondria were shorter and wider than controls, and hyperthyroid mitochondria longer but of similar width. Mitochondrial volume per unit cell volume was virtually unchanged in hypo- and hyperthyroid animals. The most striking changes were a decrease in the area of the inner membrane plus cristae in thyroidectomized rats, and a substantial increase in membrane area after thyroxine administration. The biochemical and electron micrographic results indicate that, in rat liver, thyroid hormone administration leads to a selective increase in the relative amount of mitochondrial inner membranes, with little or no change in the mitochondrial volume per unit cell volume, or in total mitochondrial protein per unit total cell protein.
在给甲状腺切除的大鼠和正常大鼠注射甲状腺素后，已对肝匀浆和纯化线粒体中的线粒体内膜标志物心磷脂和细胞色素α的浓度进行了测量。生化结果已与肝脏切片中的肝细胞以及分离的线粒体沉淀的体视学电子显微镜分析相关联。在给甲状腺切除的大鼠隔日注射20微克甲状腺素，以及给正常大鼠隔日注射300微克甲状腺素后，匀浆和线粒体中心磷脂浓度以及线粒体细胞色素α浓度均呈渐进性和平行性增加。电子显微镜测量显示，在不同甲状腺状态下，线粒体的形状和嵴的数量存在明显差异。甲状腺功能减退的线粒体比对照组更短更宽，而甲状腺功能亢进的线粒体更长但宽度相似。甲状腺功能减退和亢进的动物中，单位细胞体积的线粒体体积实际上没有变化。最显著的变化是甲状腺切除的大鼠内膜加嵴的面积减少，以及注射甲状腺素后膜面积大幅增加。生化和电子显微镜结果表明，在大鼠肝脏中，给予甲状腺激素会导致线粒体内膜相对量的选择性增加，而单位细胞体积的线粒体体积或单位总细胞蛋白中的线粒体总蛋白几乎没有变化。
