Francišković Marina, Gonzalez-Pérez Raquel, Orčić Dejan, Sánchez de Medina Fermín, Martínez-Augustin Olga, Svirčev Emilija, Simin Nataša, Mimica-Dukić Neda
University of Novi Sad, Faculty of Sciences, Department of Chemistry, Biochemistry and Environmental Protection, Trg Dositeja Obradovića 3, 21000, Novi Sad, Serbia.
Department of Pharmacology, CIBERehd, School of Pharmacy, University of Granada, Granada, Spain.
Phytother Res. 2017 Aug;31(8):1183-1191. doi: 10.1002/ptr.5836. Epub 2017 May 24.
The purpose of this work was to determine the chemical profile of stinging nettle and to provide an insight into the mechanisms by which it ameliorates the immune response. Qualitative and quantitative liquid chromatography tandem mass spectrometry analyses indicated that phenolic acids (5-O-caffeoylquinic acid as dominant) and flavonol glycosides (rutin, isoquercitrin, and kaempferol 3-O-glucoside) are present in the aerial parts, while lignans (secoisolariciresinol, 9,9'-bisacetyl-neo-olivil and their glucosides) were detected in the root. Herb and root extracts expressed selective inhibition toward cyclooxygenase and lipoxygenase branches in human platelets: root extracts were better at inhibiting thromboxane production, while herb extracts were more specific toward inhibition of 12-lipoxygenase pathway. Stinging nettle extracts mildly increased monocyte chemoattractant protein-1 and growth-related oncogene release from nonstimulated intestinal epithelial cells, stimulating MyD88/NF-κB/p38 signaling, hence preserving the epithelial integrity and enhancing intestinal steady-state defense. Additionally, root extract reduced lipopolysaccharide-induced monocyte chemoattractant protein-1/growth-related oncogene secretion and cyclooxygenase-2 expression in intestinal epithelial cells, thus showing the potential protective effect against tissue damage caused by inflammation processes. These observations suggest that stinging nettle is an interesting candidate for the development of phytopharmaceuticals or dietary supplements for cotreatment of various inflammatory diseases, particularly inflammatory bowel diseases. Copyright © 2017 John Wiley & Sons, Ltd.
这项工作的目的是确定荨麻的化学特征，并深入了解其改善免疫反应的机制。定性和定量液相色谱串联质谱分析表明，地上部分含有酚酸（以5-O-咖啡酰奎尼酸为主）和黄酮醇苷（芦丁、异槲皮苷和山奈酚3-O-葡萄糖苷），而根部检测到木脂素（开环异落叶松脂素、9,9'-双乙酰新橄榄脂素及其糖苷）。草药和根部提取物对人血小板中的环氧合酶和脂氧合酶分支表现出选择性抑制作用：根部提取物在抑制血栓素生成方面效果更好，而草药提取物对12-脂氧合酶途径的抑制更具特异性。荨麻提取物轻度增加了非刺激肠道上皮细胞中单核细胞趋化蛋白-1和生长相关癌基因的释放，刺激了MyD88/NF-κB/p38信号通路，从而维持上皮完整性并增强肠道稳态防御。此外，根部提取物减少了脂多糖诱导的肠道上皮细胞中单核细胞趋化蛋白-1/生长相关癌基因的分泌和环氧合酶-2的表达，因此显示出对炎症过程引起的组织损伤的潜在保护作用。这些观察结果表明，荨麻是开发用于联合治疗各种炎症性疾病，特别是炎症性肠病的植物药或膳食补充剂的一个有吸引力的候选者。版权所有© 2017约翰威立父子有限公司。
