Laboratory of Molecular and Preventive Dentistry, Department of Preventive Dentistry, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Huangpu District, Shanghai 200011, China.
Department of Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan; Endowed Department of International Oral Health Science (affiliated with Department of Translational Research), School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama-shi, Kanagawa 230-0063, Japan.
Dent Mater. 2021 Aug;37(8):1260-1272. doi: 10.1016/j.dental.2021.04.007. Epub 2021 May 6.
We aimed to evaluate morphological, mechanical and chemical characteristics at resin-dentin interfaces using multiple combinations of adhesives and resins after a short-term biofilm-induced degradation.
Cervical cavities were prepared in bovine incisors, treated by Clearfil SE Bond 2 (SE) or FL-Bond II (FL), restored by Clearfil Majesty ES Flow (ES) or Beautifil Flow Plus (BFP) and grouped into SE-ES, SE-BFP, FL-ES and FL-BFP. After biofilm challenge, interfacial gaps and dentin wall lesions were examined by optical coherence tomography (OCT). Gap depth (GD), gap pattern scale (GPS) and dentin wall lesion depth (WLD) were evaluated from confocal laser scanning microscope. Microhardness of dentin lesions was measured with a Vickers microhardness tester. Chemical elements in resins and dentin wall lesions were analyzed by scanning electron microscopy and energy dispersive X-ray spectrometry (SEM/EDS). Morphological structures of interfacial gaps were observed by SEM.
OCT could detect adhesive-dentin-bonded and adhesive-dentin-debonded gaps. SE-containing groups showed significantly lower GPS than FL-containing groups. FL-BFP showed significantly lower WLD than FL-ES. Microhardness of dentin wall lesions was higher than that of outer lesions and they showed significant differences in FL-BFP. SE-BFP showed a lower GPS curve and higher intensities of Ca and P in the upper half of dentin wall lesions than other groups. From SEM, microgaps between filler and matrix, break and loss of matrix, separation of adhesive matrix with hybrid layer occurred at interfacial gaps.
The morphological, mechanical and chemical characteristics of resin-dentin interfacial degradation depend on the component and chemistry of restorative materials.
本研究旨在评估短期生物膜诱导降解后，不同粘接剂和树脂组合在树脂-牙本质界面的形态、力学和化学特性。
在牛切牙的颈部制备腔，分别用 Clearfil SE Bond 2（SE）或 FL-Bond II（FL）处理，用 Clearfil Majesty ES Flow（ES）或 Beautifil Flow Plus（BFP）修复，并分为 SE-ES、SE-BFP、FL-ES 和 FL-BFP 组。生物膜挑战后，采用光学相干断层扫描（OCT）观察界面间隙和牙本质壁损伤。采用共聚焦激光扫描显微镜评估间隙深度（GD）、间隙模式尺度（GPS）和牙本质壁损伤深度（WLD）。采用维氏硬度计测量牙本质损伤的显微硬度。采用扫描电子显微镜和能谱仪（SEM/EDS）分析树脂和牙本质壁损伤中的化学元素。采用扫描电子显微镜观察界面间隙的形态结构。
OCT 可检测粘接剂-牙本质-结合和粘接剂-牙本质-脱结合间隙。含 SE 的组 GPS 明显低于含 FL 的组。FL-BFP 的 WLD 明显低于 FL-ES。牙本质壁损伤的显微硬度高于外损伤，FL-BFP 差异显著。SE-BFP 在上半部分的 GPS 曲线较低，牙本质壁损伤中 Ca 和 P 的强度较高。从 SEM 上看，填充物和基质之间的微间隙、基质的断裂和损失、以及与混合层分离的粘接剂基质都出现在界面间隙。
树脂-牙本质界面降解的形态、力学和化学特性取决于修复材料的成分和化学性质。
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