Mišković Jovana, Karaman Maja, Rašeta Milena, Krsmanović Nenad, Berežni Sanja, Jakovljević Dragica, Piattoni Federica, Zambonelli Alessandra, Gargano Maria Letizia, Venturella Giuseppe
Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, TrgDositejaObradovića 2, 21000 Novi Sad, Serbia.
Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 3, 21000 Novi Sad, Serbia.
J Fungi (Basel). 2021 Feb 4;7(2):115. doi: 10.3390/jof7020115.
In recent years, fungi have been recognized as producers of acetylcholinesterase (AChE) inhibitors, agents important for the prevention of Alzheimer's disease (AD). This study aimed to examine the AChE inhibitory, the antioxidative and antibacterial activity of two different strains that originated from Serbia (SRB) and Italy (IT). Submerged cultivation of grown mycelia (M) and fermentation broth (F) of ethanol (EtOH) and polysaccharide (PSH) extracts lasted for 7, 14, 21 and 28 days. For AChE activity Ellman method was performed, while for antioxidative activity, sevendifferent assays were conducted: DPPH, ABTS, FRAP, SOA, OH, NO together with total phenolic content. Antimicrobial screen, LC-MS/MS technique and FTIR measurements were performed. Different isolates exhibited different AChE activity, with PSH being the strongest (SRB, M, 28 days IC 79.73 ± 26.34 µg/mL), while in EtOH extracts, IT stood out (F, 14 days, IC 0.8 ± 0.6 µg/mL). PSH extracts (7 days) exhibit significant antioxidative activity (AO), opposite to EtOH extracts where 14 and 21days periods stood out. Only tw extracts showed antibacterial activity. Following LC-MS/MS analysis -hydroxybenzoic and gallic acids were the most abundant phenolics. PSH extracts demonstrated remarkable results, making this study debut and introducing as a valuable resource of AChE inhibitors.
近年来，真菌已被公认为是乙酰胆碱酯酶（AChE）抑制剂的生产者，而乙酰胆碱酯酶抑制剂是预防阿尔茨海默病（AD）的重要药物。本研究旨在检测源自塞尔维亚（SRB）和意大利（IT）的两种不同菌株的AChE抑制活性、抗氧化活性和抗菌活性。对乙醇（EtOH）提取物和多糖（PSH）提取物的菌丝体（M）和发酵液（F）进行液体深层培养，持续7、14、21和28天。采用Ellman法检测AChE活性，同时进行七种不同的抗氧化活性检测：DPPH、ABTS、FRAP、SOA、OH、NO以及总酚含量检测。进行了抗菌筛选、LC-MS/MS技术和傅里叶变换红外光谱（FTIR）测量。不同的分离物表现出不同的AChE活性，PSH的活性最强（SRB，M，28天，IC₅₀为79.73±26.34μg/mL），而在EtOH提取物中，IT菌株表现突出（F，14天，IC₅₀为0.8±0.6μg/mL）。PSH提取物（7天）表现出显著的抗氧化活性（AO）, 这与EtOH提取物中14天和21天表现突出的情况相反。只有两种提取物显示出抗菌活性。经过LC-MS/MS分析，对羟基苯甲酸和没食子酸是含量最丰富的酚类物质。PSH提取物显示出显著的结果，使本研究首次亮相，并将其作为AChE抑制剂的宝贵资源引入。
