Sun Huan, Ren Yan, Hou Weijie, Li Lin, Zeng Fanqi, Li Sisi, Ma Yongfen, Liu Xiao, Chen She, Zhang Zhiyuan
College of Life Sciences, Beijing Normal University, Beijing 100875, China and National Institute of Biological Sciences (NIBS), Beijing 102206, China.
National Institute of Biological Sciences (NIBS), Beijing 102206, China.
Chem Commun (Camb). 2016 Aug 11;52(67):10225-8. doi: 10.1039/c6cc04030f.
A new and efficient method focusing on probe-modified peptides was developed to identify the target protein and modification site of a hit compound or a drug. This method exhibited high click conjugation efficiency and few false-positive results. The modification site further facilitated target validation, biological mechanism study and new indications exploration.
