硫嘌呤的微生物代谢：一种测量硫鸟嘌呤核苷酸的方法。

Microbial metabolism of thiopurines: A method to measure thioguanine nucleotides.

作者信息

Movva Ramya, Lobb Michael, Ó Cuív Páraic, Florin Timothy H J, Duley John A, Oancea Iulia

机构信息

Immunity Infection and Inflammation Program, Mater Research Institute-University of Queensland, Brisbane, Queensland 4101, Australia; Translational Research Institute, 37 Kent Street, Woolloongabba, Queensland 4102, Australia; School of Pharmacy, Griffith University, Queensland 4125, Australia.

Inflammatory Diseases Biology & Therapeutics Program, Mater Research Institute-University of Queensland, Brisbane, Queensland 4101, Australia.

出版信息

J Microbiol Methods. 2016 Sep;128:102-107. doi: 10.1016/j.mimet.2016.07.017. Epub 2016 Jul 19.

DOI:10.1016/j.mimet.2016.07.017

PMID:27444548

Abstract

Thiopurines are anti-inflammatory prodrugs. We hypothesised that bacteria may contribute to conversion to active drug. Escherichia coli strain DH5α was evaluated to determine whether it could metabolise the thiopurine drugs, thioguanine or mercaptopurine, to thioguanine nucleotides. A rapid and reliable high performance liquid chromatography (ultraviolet detection) method was developed to quantify indirectly thioguanine nucleotides, by measuring thioguanine nucleoside.

摘要

硫嘌呤是抗炎前体药物。我们推测细菌可能有助于其转化为活性药物。对大肠杆菌DH5α菌株进行评估，以确定它是否能将硫嘌呤药物硫鸟嘌呤或巯嘌呤代谢为硫鸟嘌呤核苷酸。通过测量硫鸟嘌呤核苷，开发了一种快速可靠的高效液相色谱（紫外检测）方法来间接定量硫鸟嘌呤核苷酸。