Endocrine Research Unit, Mayo School of Graduate Medical Education, 200 First Street SW, Rochester, MN, 55905, USA.
Immunochemical Laboratory, 200 First Street SW, Rochester, MN, 55905, USA.
Endocrine. 2016 Dec;54(3):671-680. doi: 10.1007/s12020-016-1041-3. Epub 2016 Jul 22.
Available data associate lipids concentrations in men with body mass index, anabolic steroids, age, and certain cytokines. Data were less clear in women, especially across the full adult lifespan, and when segmented by premenopausal and postmenopausal status.
120 healthy women (60 premenopausal and 60 postmenopausal) in Olmsted County, MN, USA, a stable well studied clinical population. Dependent variables: measurements of 10 h fasting high-density lipoprotein cholesterol, total cholesterol, low-density lipoprotein cholesterol, and triglycerides.
testosterone, estrone, estradiol, 5-alpha-dihydrotestosterone, and sex-hormone binding globulin (by mass spectrometry); insulin, glucose, and albumin; abdominal visceral, subcutaneous, and total abdominal fat [abdominal visceral fat, subcutaneous fat, total abdominal fat by computerized tomography scan]; and a panel of cytokines (by enzyme-linked immunosorbent assay). Multivariate forward-selection linear-regression analysis was applied constrained to P < 0.01. Lifetime data: High-density lipoprotein cholesterol was correlated jointly with age (P < 0.0001, positively), abdominal visceral fat (P < 0.0001, negatively), and interleukin-6 (0.0063, negatively), together explaining 28.1 % of its variance (P = 2.3 × 10). Total cholesterol was associated positively with multivariate age only (P = 6.9 × 10, 9.3 % of variance). Triglycerides correlated weakly with sex-hormone binding globulin (P = 0.0115), and strongly with abdominal visceral fat (P < 0.0001), and interleukin-6 (P = 0.0016) all positively (P = 1.6 × 10, 38.9 % of variance). Non high-density lipoprotein cholesterol and low-density lipoprotein cholesterol correlated positively with both total abdominal fat and interleukin-8 (P = 2.0 × 10, 16.9 % of variance; and P = 0.0031, 9.4 % of variance, respectively). Premenopausal vs. postmenopausal comparisons identified specific relationships that were stronger in premenopausal than postmenopausal individuals, and vice versa. Age was a stronger correlate of low-density lipoprotein cholesterol; interleukin-6 of triglycerides and high-density lipoprotein; and both sex-hormone binding globulin and total abdominal fat of non high-density lipoprotein cholesterol in premenopausal than postmenopausal women. Conversely, sex-hormone binding globulin, abdominal visceral fat, interleukin-8, adiponectin were stronger correlates of triglycerides; abdominal visceral fat, and testosterone of high-density lipoprotein cholesterol; and age of both non high-density lipoprotein and low-density lipoprotein in postmenopausal than premenopausal women. Our data delineate correlations of total abdominal fat and interleukin-8 (both positively) with non high-density lipoprotein cholesterol and low-density lipoprotein cholesterol in healthy women across the full age range of 21-79 years along with even more specific associations in premenopausal and postmenopausal individuals. Whether some of these outcomes reflect causal relationships would require longitudinal and interventional or genetic studies.
本研究旨在探讨男性身体质量指数、合成代谢类固醇、年龄和某些细胞因子与血脂浓度的关系。然而，女性的数据相对较少，尤其是在整个成年期，并且在绝经前和绝经后状态下的数据也不够清晰。
研究对象为美国明尼苏达州奥姆斯特德县的 120 名健康女性（60 名绝经前和 60 名绝经后），这是一个稳定的、经过充分研究的临床人群。研究的依赖变量为 10 小时禁食后的高密度脂蛋白胆固醇、总胆固醇、低密度脂蛋白胆固醇和甘油三酯的测量值。
研究的独立变量包括睾酮、雌酮、雌二醇、5α-二氢睾酮和性激素结合球蛋白（通过质谱法）；胰岛素、葡萄糖和白蛋白；腹部内脏脂肪、皮下脂肪和总腹部脂肪（通过计算机断层扫描）；以及细胞因子的面板（通过酶联免疫吸附测定法）。应用多元向前选择线性回归分析，约束 P 值<0.01。终身数据：高密度脂蛋白胆固醇与年龄呈正相关（P<0.0001），与腹部内脏脂肪呈负相关（P<0.0001），与白细胞介素-6 呈负相关（0.0063），共同解释其 28.1%的变异（P=2.3×10）。总胆固醇仅与多元年龄呈正相关（P=6.9×10，9.3%的变异）。甘油三酯与性激素结合球蛋白呈弱相关（P=0.0115），与腹部内脏脂肪呈强相关（P<0.0001），与白细胞介素-6 呈正相关（P=0.0016），所有这些相关性均呈强正相关（P=1.6×10，38.9%的变异）。非高密度脂蛋白胆固醇和低密度脂蛋白胆固醇与总腹部脂肪和白细胞介素-8 呈正相关（P=2.0×10，16.9%的变异；P=0.0031，9.4%的变异）。绝经前与绝经后比较发现，在绝经前个体中，特定的关系比绝经后个体更强，反之亦然。年龄与低密度脂蛋白胆固醇的相关性更强；白细胞介素-6 与甘油三酯和高密度脂蛋白的相关性更强；而性激素结合球蛋白和总腹部脂肪与非高密度脂蛋白胆固醇的相关性在绝经前女性中比绝经后女性更强。相反，性激素结合球蛋白、腹部内脏脂肪、白细胞介素-8 和脂联素与甘油三酯的相关性更强；腹部内脏脂肪和睾酮与高密度脂蛋白胆固醇的相关性更强；年龄与非高密度脂蛋白和低密度脂蛋白在绝经后女性中比绝经前女性更强。我们的数据描绘了总腹部脂肪和白细胞介素-8（均为正相关）与健康女性全年龄段 21-79 岁之间的非高密度脂蛋白胆固醇和低密度脂蛋白胆固醇之间的相关性，以及绝经前和绝经后个体中更具体的关联。这些结果中的一些是否反映了因果关系，需要进行纵向和干预或遗传研究。
