• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

二苯甲酮合酶和查尔酮合酶在不同发育阶段贯叶连翘叶片的叶肉细胞中积累。

Benzophenone Synthase and Chalcone Synthase Accumulate in the Mesophyll of Hypericum perforatum Leaves at Different Developmental Stages.

作者信息

Belkheir Asma K, Gaid Mariam, Liu Benye, Hänsch Robert, Beerhues Ludger

机构信息

Institute of Pharmaceutical Biology, Technische Universität Braunschweig Braunschweig, Germany.

Institute of Plant Biology, Technische Universität Braunschweig Braunschweig, Germany.

出版信息

Front Plant Sci. 2016 Jun 29;7:921. doi: 10.3389/fpls.2016.00921. eCollection 2016.

DOI:10.3389/fpls.2016.00921
PMID:27446151
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4926534/
Abstract

The active medicinal constituents in Hypericum perforatum, used to treat depression and skin irritation, include flavonoids and xanthones. The carbon skeletons of these compounds are formed by chalcone synthase (CHS) and benzophenone synthase (BPS), respectively. Polyclonal antisera were raised against the polyketide synthases from Hypericum androsaemum and their IgG fractions were isolated. Immunoblotting and immunotitration were used to test the IgGs for crossreactivity and monospecificity in H. perforatum leaf protein extract. Immunofluorescence localization revealed that both CHS and BPS are located in the mesophyll. The maximum fluorescence levels were observed in approx. 0.5 and 1 cm long leaves, respectively. The fluorescence intensity observed for CHS significantly exceeded that for BPS. Using histochemical staining, flavonoids were detected in the mesophyll, indicating that the sites of biosynthesis and accumulation coincide. Our results help understand the biosynthesis and underlying regulation of active H. perforatum constituents.

摘要

贯叶连翘中用于治疗抑郁症和皮肤刺激的活性药用成分包括黄酮类化合物和呫吨酮。这些化合物的碳骨架分别由查尔酮合酶（CHS）和二苯甲酮合酶（BPS）形成。制备了针对红果金丝桃聚酮合酶的多克隆抗血清，并分离出其IgG组分。采用免疫印迹和免疫滴定法检测IgG在贯叶连翘叶片蛋白提取物中的交叉反应性和单特异性。免疫荧光定位显示，CHS和BPS均位于叶肉中。分别在约0.5厘米和1厘米长的叶片中观察到最大荧光水平。观察到的CHS荧光强度显著超过BPS。通过组织化学染色，在叶肉中检测到黄酮类化合物，表明生物合成和积累部位一致。我们的结果有助于了解贯叶连翘活性成分的生物合成及潜在调控机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c922/4926534/5254496d76a4/fpls-07-00921-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c922/4926534/9b46702ef205/fpls-07-00921-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c922/4926534/4f9e9eb620e9/fpls-07-00921-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c922/4926534/8acfd7e71d36/fpls-07-00921-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c922/4926534/a6d89f430168/fpls-07-00921-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c922/4926534/5f783134f3dd/fpls-07-00921-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c922/4926534/5254496d76a4/fpls-07-00921-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c922/4926534/9b46702ef205/fpls-07-00921-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c922/4926534/4f9e9eb620e9/fpls-07-00921-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c922/4926534/8acfd7e71d36/fpls-07-00921-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c922/4926534/a6d89f430168/fpls-07-00921-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c922/4926534/5f783134f3dd/fpls-07-00921-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c922/4926534/5254496d76a4/fpls-07-00921-g006.jpg

相似文献

1
Benzophenone Synthase and Chalcone Synthase Accumulate in the Mesophyll of Hypericum perforatum Leaves at Different Developmental Stages.二苯甲酮合酶和查尔酮合酶在不同发育阶段贯叶连翘叶片的叶肉细胞中积累。
Front Plant Sci. 2016 Jun 29;7:921. doi: 10.3389/fpls.2016.00921. eCollection 2016.
2
Benzophenone synthase and chalcone synthase from Hypericum androsaemum cell cultures: cDNA cloning, functional expression, and site-directed mutagenesis of two polyketide synthases.来自红果金丝桃细胞培养物的二苯甲酮合酶和查尔酮合酶：两种聚酮合酶的cDNA克隆、功能表达及定点诱变
Plant J. 2003 Jun;34(6):847-55. doi: 10.1046/j.1365-313x.2003.01771.x.
3
Exodermis and endodermis are the sites of xanthone biosynthesis in Hypericum perforatum roots.黄烷酮生物合成发生于贯叶连翘根的外表皮和内皮层。
New Phytol. 2018 Feb;217(3):1099-1112. doi: 10.1111/nph.14929. Epub 2017 Dec 6.
4
Final report on the safety assessment of Hypericum perforatum extract and Hypericum perforatum oil.贯叶连翘提取物和贯叶连翘油安全性评估最终报告。
Int J Toxicol. 2001;20 Suppl 2:31-9. doi: 10.1080/10915810160233749.
5
Octaketide-producing type III polyketide synthase from Hypericum perforatum is expressed in dark glands accumulating hypericins.贯叶连翘中产生八酮化合物的III型聚酮合酶在积累金丝桃素的深色腺体内表达。
FEBS J. 2008 Sep;275(17):4329-42. doi: 10.1111/j.1742-4658.2008.06576.x. Epub 2008 Jul 21.
6
Biosynthesis of the hyperforin skeleton in Hypericum calycinum cell cultures.金丝桃细胞培养物中金丝桃素骨架的生物合成。
Phytochemistry. 2005 Jan;66(2):139-45. doi: 10.1016/j.phytochem.2004.11.003.
7
Preparation of a polyclonal antibody against hypericin synthase and localization of the enzyme in red-pigmented Hypericum perforatum L. plantlets.抗金丝桃素合酶多克隆抗体的制备及该酶在红色金丝桃组培苗中的定位
Acta Biochim Pol. 2012;59(4):639-45. Epub 2012 Nov 16.
8
Optimization of protein extraction from Hypericum perforatum tissues and immunoblotting detection of Hyp-1 at different stages of leaf development.优化贯叶连翘组织中的蛋白质提取，并在叶片发育的不同阶段检测 Hyp-1 的免疫印迹。
Mol Biotechnol. 2010 Nov;46(3):219-26. doi: 10.1007/s12033-010-9299-9.
9
Differential expression of benzophenone synthase and chalcone synthase in Hypericum sampsonii.元宝草中苯甲酮合酶和查尔酮合酶的差异表达
Nat Prod Commun. 2012 Dec;7(12):1615-8.
10
Xanthone biosynthesis in Hypericum perforatum cells provides antioxidant and antimicrobial protection upon biotic stress.贯叶连翘细胞中的呫吨酮生物合成在生物胁迫时提供抗氧化和抗菌保护。
Phytochemistry. 2009 Jan;70(1):60-8. doi: 10.1016/j.phytochem.2008.10.016. Epub 2008 Dec 4.

引用本文的文献

1
Phytochemical profiling and antioxidant capacity of Achillea Santolina at different phenological stages.不同物候期蓍草的植物化学特征分析及抗氧化能力研究
Sci Rep. 2025 Jul 17;15(1):25885. doi: 10.1038/s41598-025-11873-3.
2
Phytochemical composition and in vitro antioxidant and antimicrobial activities of Bersama abyssinica F. seed extracts.Bersama abyssinica F. 种子提取物的植物化学成分及体外抗氧化和抗菌活性。
Sci Rep. 2024 Mar 15;14(1):6345. doi: 10.1038/s41598-024-56659-1.
3
Anabolic metabolism of autotoxic substance coumarins in plants.

本文引用的文献

1
Bifunctional CYP81AA proteins catalyse identical hydroxylations but alternative regioselective phenol couplings in plant xanthone biosynthesis.双功能 CYP81AA 蛋白在植物黄烷酮生物合成中催化相同的羟基化反应，但具有不同的酚羟基偶联区域选择性。
Nat Commun. 2016 May 5;7:11472. doi: 10.1038/ncomms11472.
2
Molecular Cloning and Characterization of a Xanthone Prenyltransferase from Hypericum calycinum Cell Cultures.金丝桃细胞培养物中一种呫吨酮类异戊烯基转移酶的分子克隆与特性分析
Molecules. 2015 Aug 27;20(9):15616-30. doi: 10.3390/molecules200915616.
3
Xanthones from roots, hairy roots and cell suspension cultures of selected Hypericum species and their antifungal activity against Candida albicans.
植物中自毒性物质香豆素的合成代谢。
PeerJ. 2023 Dec 6;11:e16508. doi: 10.7717/peerj.16508. eCollection 2023.
4
Transcriptomic and Metabolomic Analyses Reveal Differences in Flavonoid Pathway Gene Expression Profiles between Two Varieties during Vernalization.转录组学和代谢组学分析揭示了两个品种在春化过程中类黄酮途径基因表达谱的差异。
Int J Mol Sci. 2023 Jul 3;24(13):11039. doi: 10.3390/ijms241311039.
5
Influence of geographic origin and tissue type on the medicinal chemical compounds of .地理起源和组织类型对. 的药用化学成分的影响。
PeerJ. 2023 Jun 6;11:e15484. doi: 10.7717/peerj.15484. eCollection 2023.
6
Xanthones: Biosynthesis and Trafficking in Plants, Fungi and Lichens.氧杂蒽酮：植物、真菌和地衣中的生物合成与转运
Plants (Basel). 2023 Feb 4;12(4):694. doi: 10.3390/plants12040694.
7
Relationships between Agronomic Traits of Moringa Accessions and In Vitro Gas Production Characteristics of a Test Feed Incubated with or without Moringa Plant Leaf Extracts.辣木种质资源农艺性状与添加或不添加辣木叶提取物的试验饲料体外产气特性之间的关系。
Plants (Basel). 2022 Oct 28;11(21):2901. doi: 10.3390/plants11212901.
8
A Comparison of the Flavonoid Biosynthesis Mechanisms of Species by Analyzing the Transcriptome and Metabolome.通过分析转录组和代谢组比较 种黄酮类生物合成机制。
Int J Mol Sci. 2022 Oct 9;23(19):11980. doi: 10.3390/ijms231911980.
9
Xanthone Biosynthetic Pathway in Plants: A Review.植物中的氧杂蒽酮生物合成途径：综述
Front Plant Sci. 2022 Apr 8;13:809497. doi: 10.3389/fpls.2022.809497. eCollection 2022.
10
Biotransformation of Benzoate to 2,4,6-Trihydroxybenzophenone by Engineered .苯甲酸到 2,4,6-三羟基二苯甲酮的生物转化由工程菌.
Molecules. 2021 May 8;26(9):2779. doi: 10.3390/molecules26092779.
黄烷酮类化合物来源于某些贯叶连翘属植物的根、发根和细胞悬浮培养物及其对白色念珠菌的抗真菌活性。
Plant Cell Rep. 2015 Nov;34(11):1953-62. doi: 10.1007/s00299-015-1842-5. Epub 2015 Jul 21.
4
On the localization of enzymes related to flavonoid metabolism in sections and tissues of oat primary leaves.在燕麦初生叶片的切片和组织中与类黄酮代谢相关的酶的定位。
Planta. 1977 Jan;137(1):49-52. doi: 10.1007/BF00394434.
5
Tissue-distribution of secondary phenolic biosynthesis in developing primary leaves of Avena sativa L.燕麦发育主叶中次生酚类生物合成的组织分布
Planta. 1986 Feb;167(2):196-205. doi: 10.1007/BF00391415.
6
Chalcone synthases from spinach (Spinacia oleracea L.) : II. Immunofluorescence and immunogold localization.菠菜查尔酮合酶的研究 II. 免疫荧光和免疫胶体金定位。
Planta. 1988 Dec;173(4):544-53. doi: 10.1007/BF00958968.
7
Chalcone synthases from spinach (Spinacia oleracea L.) : I. Purification, peptide patterns, and immunological properties of different forms.菠菜查尔酮合酶的研究：I. 不同形式的纯化、肽图谱和免疫学特性。
Planta. 1988 Dec;173(4):532-43. doi: 10.1007/BF00958967.
8
Differential regulation and tissue-specific distribution of enzymes of phenylpropanoid pathways in developing parsley seedlings.在发育中的欧芹幼苗中苯丙烷途径的酶的差异调节和组织特异性分布。
Planta. 1988 Dec;173(4):453-8. doi: 10.1007/BF00958957.
9
Topical application of St. John's wort (Hypericum perforatum).圣约翰草（贯叶连翘）的局部应用。
Planta Med. 2014 Feb;80(2-3):109-20. doi: 10.1055/s-0033-1351019. Epub 2013 Nov 8.
10
Differential expression of benzophenone synthase and chalcone synthase in Hypericum sampsonii.元宝草中苯甲酮合酶和查尔酮合酶的差异表达
Nat Prod Commun. 2012 Dec;7(12):1615-8.