College of Agronomy, Henan Agricultural University/ National Engineering Research Center for Wheat/ Co-construction State Key Laboratory of Wheat and Maize Crop Science/ Collaborative Innovation Center of Henan Grain Crops, 15 Longzihu College District, Zhengzhou, 450046, China.
College of Plant Protection, Henan Agricultural University, Zhengzhou, 450002, Henan, China.
BMC Plant Biol. 2021 Feb 8;21(1):82. doi: 10.1186/s12870-021-02853-6.
Fusarium crown rot is major disease in wheat. However, the wheat defense mechanisms against this disease remain poorly understood.
Using tandem mass tag (TMT) quantitative proteomics, we evaluated a disease-susceptible (UC1110) and a disease-tolerant (PI610750) wheat cultivar inoculated with Fusarium pseudograminearum WZ-8A. The morphological and physiological results showed that the average root diameter and malondialdehyde content in the roots of PI610750 decreased 3 days post-inoculation (dpi), while the average number of root tips increased. Root vigor was significantly increased in both cultivars, indicating that the morphological, physiological, and biochemical responses of the roots to disease differed between the two cultivars. TMT analysis showed that 366 differentially expressed proteins (DEPs) were identified by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment in the two comparison groups, UC1110_3dpi/UC1110_0dpi (163) and PI610750_3dpi/PI610750_0dpi (203). It may be concluded that phenylpropanoid biosynthesis (8), secondary metabolite biosynthesis (12), linolenic acid metabolites (5), glutathione metabolism (8), plant hormone signal transduction (3), MAPK signaling pathway-plant (4), and photosynthesis (12) contributed to the defense mechanisms in wheat. Protein-protein interaction network analysis showed that the DEPs interacted in both sugar metabolism and photosynthesis pathways. Sixteen genes were validated by real-time quantitative polymerase chain reaction and were found to be consistent with the proteomics data.
The results provided insight into the molecular mechanisms of the interaction between wheat and F. pseudograminearum.
镰刀菌顶腐病是小麦的主要病害。然而，小麦对这种疾病的防御机制仍知之甚少。
使用串联质量标签（TMT）定量蛋白质组学，我们评估了接种禾谷镰刀菌 WZ-8A 的感病（UC1110）和抗病（PI610750）小麦品种。形态和生理结果表明，PI610750 根部的平均根直径和丙二醛含量在接种后 3 天（dpi）下降，而根尖的平均数量增加。两个品种的根系活力均显著增加，表明两个品种根系对疾病的形态、生理和生化反应不同。TMT 分析显示，在两个比较组 UC1110_3dpi/UC1110_0dpi（163 个）和 PI610750_3dpi/PI610750_0dpi（203 个）中，通过基因本体论和京都基因与基因组百科全书富集共鉴定出 366 个差异表达蛋白（DEPs）。可以得出结论，苯丙烷生物合成（8）、次生代谢物生物合成（12）、亚油酸代谢物（5）、谷胱甘肽代谢（8）、植物激素信号转导（3）、MAPK 信号通路-植物（4）和光合作用（12）有助于小麦的防御机制。蛋白质-蛋白质相互作用网络分析表明，DEPs 在糖代谢和光合作用途径中相互作用。通过实时定量聚合酶链反应验证了 16 个基因，发现与蛋白质组学数据一致。
这些结果为小麦与禾谷镰刀菌相互作用的分子机制提供了深入了解。
