Zheng Lin-Feng, Sun Wen-Yong
Department of Pathology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China.
Biomed Rep. 2016 Aug;5(2):159-164. doi: 10.3892/br.2016.705. Epub 2016 Jun 23.
Bladder cancer is the most common cancer of the urinary tract. A quarter of bladder cancer patients presenting with muscle-invasive bladder cancer (MIBC) suffer significant morbidity and succumb to the disease. MicroRNA (miRNA) from tissue, urine or blood samples of MIBC patients have been demonstrated to differ from healthy individuals, and possibly have diagnostic value. The aim of the present meta-analysis was to access the overall diagnostic accuracy comprehensively and quantitatively. Systematic searching in PubMed, Web of Science, Embase and Chinese National Knowledge Infrastructure database was conducted. The pooled sensitivity, specificity, positive and negative likelihood ratios (PLR and NLR) and diagnostic odds ratio (DOR) were calculated via the random effects model to evaluate the overall test performance. Deeks' funnel plot asymmetry test was used to test the publication bias. A total of 10 studies were included in the meta-analysis, with a total of 577 patients and 412 controls. The pooled sensitivity and specificity were 0.78 [95% confidence interval (CI), 0.69-0.86] and 0.77 (95% CI, 0.72-0.81), respectively. The pooled PLR was 2.9 (95% CI, 2.1-3.8), the NLR was 0.31 (95% CI, 0.27-0.35), the DOR was 7 (95% CI, 4-13) and the pooled AUC was 0.80 (95% CI, 0.69-0.87). In conclusion, the current miRNA assays support their use as markers for MIBC diagnosis.
膀胱癌是泌尿系统最常见的癌症。四分之一表现为肌层浸润性膀胱癌（MIBC）的患者会出现严重的发病情况并死于该疾病。已证明，MIBC患者组织、尿液或血液样本中的微小RNA（miRNA）与健康个体不同，可能具有诊断价值。本荟萃分析的目的是全面、定量地评估总体诊断准确性。我们在PubMed、Web of Science、Embase和中国国家知识基础设施数据库中进行了系统检索。通过随机效应模型计算合并敏感性、特异性、阳性和阴性似然比（PLR和NLR）以及诊断比值比（DOR），以评估总体检测性能。使用Deeks漏斗图不对称性检验来检验发表偏倚。荟萃分析共纳入10项研究，共有577例患者和412例对照。合并敏感性和特异性分别为0.78 [95%置信区间（CI），0.69 - 0.86]和0.77（95% CI，0.72 - 0.81）。合并PLR为2.9（95% CI，2.1 - 3.8），NLR为0.31（95% CI，0.27 - 0.35），DOR为7（95% CI，4 - 13），合并AUC为0.80（95% CI，0.69 - 0.87）。总之，目前的miRNA检测方法支持将其用作MIBC诊断的标志物。
