Zhang Xiaohan, Xu Miao, Su Shixin, Zhou Zeqi, Yang Hong, Zhao Suwen, Zeng Danni, Yang Kaiying, Liu Yanan, Wang Lijing, Li Jiangchao
Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China.
State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou, Guangdong 510060, P.R. China.
Mol Clin Oncol. 2016 Aug;5(2):283-288. doi: 10.3892/mco.2016.934. Epub 2016 Jun 14.
Leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5) is well-characterized as a marker of intestinal stem cells and certain types of tumor stem cells, where positive cells may develop into intestinal epithelial cells or intestinal adenomas. However, the roles of Lgr5 in the lung and in lung tumors remain unclear. An immunohistochemistry (IHC) analysis was performed to detect the expression of Lgr5 in the lung from a normal mouse. Histopathological sections of the lungs from Lgr5 heterozygous knockout mice (Lgr5) were observed following with hematoxylin and eosin. Furthermore, tissue microarrays containing tumor cores from lung cancer patients were also analyzed by IHC. Lgr5-positive cells were present in the pulmonary alveoli and bronchi of normal mice, whereas the lungs of Lgr5 mice lost their normal morphological structure compared with the lungs of the normal mice. Lgr5 was expressed in lung adenocarcinoma, however, not in squamous carcinoma, and Lgr5 expression was positively associated with tumor, node, metastasis stage. Lgr5 is expressed in normal murine lung and is associated with TNM stage in patients with lung adenocarcinoma.
富含亮氨酸重复序列的G蛋白偶联受体5（Lgr5）作为肠道干细胞和某些类型肿瘤干细胞的标志物已得到充分表征，其中阳性细胞可能发展为肠上皮细胞或肠腺瘤。然而，Lgr5在肺及肺肿瘤中的作用仍不清楚。进行了免疫组织化学（IHC）分析以检测正常小鼠肺中Lgr5的表达。用苏木精和伊红对Lgr5杂合敲除小鼠（Lgr5）的肺组织病理切片进行观察。此外，还通过IHC分析了包含肺癌患者肿瘤核心的组织微阵列。正常小鼠的肺泡和支气管中存在Lgr5阳性细胞，而与正常小鼠的肺相比，Lgr5小鼠的肺失去了正常的形态结构。Lgr5在肺腺癌中表达，但在鳞状细胞癌中不表达，且Lgr5表达与肿瘤、淋巴结、转移分期呈正相关。Lgr5在正常小鼠肺中表达，且与肺腺癌患者的TNM分期相关。
