Reyes R, Rosado A, Hernández O, Delgado N M
División de Reproducción Humana, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco.
Gamete Res. 1989 May;23(1):39-47. doi: 10.1002/mrd.1120230105.
It has been proposed that reduced glutathione (GSH) or other thiol reagents may participate in the basic mechanism by which sperm-decondensing activity is accomplished. However, in vitro, these reagents seem to be inactive and require the presence of other chemicals, usually detergents. Heparin binds specifically to the sperm membrane and provokes the decondensation of human sperm and the activation of DNA transcription and synthesis. However, the concentrations at which these effects occur seem to be higher than those expected under physiological conditions. In the present study, thiol reagents at 10 mM concentration, either alone or combined, were completely ineffective in inducing any significant nuclear decondensation after prolonged exposures (24 hr) of incubation. Heparin, 153.8 microM, was capable of inducing only a small increase in nuclear swelling. However, GSH at concentrations as low as 0.1 mM in combination with heparin induces decondensation of human sperm nuclei in vitro. When GSH concentration was kept constant at 5 mM, nuclear decondensation was induced with heparin at concentrations as low as 11.6 microM, and a maximal decondensation (90%) was obtained with only 21.6 microM of heparin. The latter is more than ten times less than the minimal active concentration of heparin used alone.
有人提出，还原型谷胱甘肽（GSH）或其他硫醇试剂可能参与了精子解凝聚活性实现的基本机制。然而，在体外，这些试剂似乎没有活性，需要其他化学物质（通常是去污剂）的存在。肝素特异性结合精子膜，引发人类精子的解凝聚以及DNA转录和合成的激活。然而，产生这些效应的浓度似乎高于生理条件下预期的浓度。在本研究中，10 mM浓度的硫醇试剂，单独使用或联合使用，在长时间孵育（24小时）后，完全无法诱导任何显著的核解凝聚。153.8 microM的肝素仅能诱导核肿胀略有增加。然而，低至0.1 mM浓度的GSH与肝素联合使用可在体外诱导人类精子核的解凝聚。当GSH浓度保持在5 mM不变时，低至11.6 microM的肝素即可诱导核解凝聚，仅21.6 microM的肝素就能实现最大解凝聚（90%）。后者比单独使用肝素的最小活性浓度低十多倍。
