Oppedal B R, Storm-Mathisen I, Kemshead J T, Brandtzaeg P
Laboratory for Immunohistochemistry and Immunopathology (LIIPAT), University of Oslo, Norway.
Hum Pathol. 1989 Aug;20(8):800-5. doi: 10.1016/0046-8177(89)90076-2.
Bone marrow examination was performed at the time of diagnosis, before initiation of therapy, and during follow-up of 14 consecutive patients with neuroblastoma who were treated at the National Hospital in Oslo. A total of 30 bone marrow specimens were examined by conventional histology, by immunohistochemistry with selected monoclonal antibodies to neuroblastoma cells (UJ 13A, UJ 167.11, PI 153/3, and A2B5) applied on trephines, and by morphologic evaluation of smears in addition to immunocytochemistry of aspirated bone marrow cells. The study confirmed the value of immunohistochemistry and immunocytochemistry for the detection of bone marrow involvement in neuroblastoma. Immunohistochemistry performed on bone marrow trephines was a slightly better adjunct than immunocytochemistry on aspirated bone marrow cells.
对在奥斯陆国家医院接受治疗的14例连续性神经母细胞瘤患者，在诊断时、开始治疗前及随访期间进行了骨髓检查。共30份骨髓标本接受了检查，检查方法包括常规组织学检查、对骨髓活检切片应用选定的抗神经母细胞瘤细胞单克隆抗体（UJ 13A、UJ 167.11、PI 153/3和A2B5）进行免疫组织化学检查、涂片的形态学评估以及抽吸骨髓细胞的免疫细胞化学检查。该研究证实了免疫组织化学和免疫细胞化学在检测神经母细胞瘤骨髓受累情况方面的价值。对骨髓活检切片进行的免疫组织化学检查作为辅助检查，其效果略优于对抽吸骨髓细胞进行的免疫细胞化学检查。
