Nagai J, Kigasawa H, Tomioka K, Koga N, Nishihira H, Nagao T
Division of Laboratory, Kanagawa Children's Medical Center, Yokohama, Japan.
Eur J Haematol. 1994 Aug;53(2):74-7. doi: 10.1111/j.1600-0609.1994.tb01868.x.
We evaluated the utility of an immunocytochemical technique employing the commercially available anti-CD56 monoclonal antibody, NKH 1. The utility and sensitivity of this technique in the detection of invasive neuroblastoma (NB) cells in the bone marrow were compared with those of Wright-Giemsa staining. The correlation coefficient for the percent NB cells detected using Wright-Giemsa staining with the percent NKH 1 immunoreactive cells was 0.78. In the analysis of specificity, this monoclonal antibody showed slight cross-reactivity with normal bone marrow cells, including macrophages, lymphocytes and osteoblasts. In the evaluation of the sensitivity of the NKH 1 immunocytochemical technique, SK-N-DZ and SK-N-SH NB cell lines were added to morphologically normal bone marrow mononuclear cells from patients without NB to the final NB cell line at concentrations of 2%, 1% and 0.1%. NB cells at the final concentration of 0.1% could be detected by the immunocytochemical technique. We conclude that the NKH 1 immunocytochemical staining technique is useful in the detection of metastatic NB cells in bone marrow.
我们评估了一种采用市售抗CD56单克隆抗体NKH 1的免疫细胞化学技术的效用。将该技术在检测骨髓中侵袭性神经母细胞瘤（NB）细胞方面的效用和敏感性与瑞氏-吉姆萨染色法进行了比较。使用瑞氏-吉姆萨染色法检测到的NB细胞百分比与NKH 1免疫反应性细胞百分比的相关系数为0.78。在特异性分析中，这种单克隆抗体与正常骨髓细胞（包括巨噬细胞、淋巴细胞和成骨细胞）表现出轻微的交叉反应性。在评估NKH 1免疫细胞化学技术的敏感性时，将SK-N-DZ和SK-N-SH NB细胞系添加到来自无NB患者的形态学正常的骨髓单个核细胞中，使最终NB细胞系的浓度分别为2%、1%和0.1%。免疫细胞化学技术能够检测到最终浓度为0.1%的NB细胞。我们得出结论，NKH 1免疫细胞化学染色技术在检测骨髓中转移性NB细胞方面是有用的。
