Chen Chun-Ju, Chen Chun-Cheng, Ding Shinn-Jyh
Institute of Oral Science, Chung Shan Medical University, Taichung City 402, Taiwan.
School of Dentistry, Chung Shan Medical University, Taichung City 402, Taiwan.
Int J Mol Sci. 2016 Jul 19;17(7):1161. doi: 10.3390/ijms17071161.
Chemotherapeutic agents have been used as an adjunct to mechanical debridement for peri-implantitis treatment. The present in vitro study evaluated and compared the effectiveness of hypochlorous acid (HOCl), sodium hypochlorite (NaOCl), and chlorhexidine (CHX) at eliminating Gram-negative (E. coli and P. gingivalis) and Gram-positive (E. faecalis and S. sanguinis) bacteria. The effect of irrigating volume and exposure time on the antimicrobial efficacy of HOCl was evaluated, and a durability analysis was completed. Live/dead staining, morphology observation, alamarBlue assay, and lipopolysaccharide (LPS) detection were examined on grit-blasted and biofilm-contaminated titanium alloy discs after treatment with the three chemotherapeutic agents. The results indicated that HOCl exhibited better antibacterial efficacy with increasing irrigating volumes. HOCl achieved greater antibacterial efficacy as treatment time was increased. A decrease in antimicrobial effectiveness was observed when HOCl was unsealed and left in contact with the air. All the irrigants showed antibacterial activity and killed the majority of bacteria on the titanium alloy surfaces of biofilm-contaminated implants. Moreover, HOCl significantly lowered the LPS concentration of P. gingivalis when compared with NaOCl and CHX. Thus, a HOCl antiseptic may be effective for cleaning biofilm-contaminated implant surfaces.
化疗药物已被用作种植体周围炎治疗中机械清创的辅助手段。本体外研究评估并比较了次氯酸（HOCl）、次氯酸钠（NaOCl）和氯己定（CHX）对消除革兰氏阴性菌（大肠杆菌和牙龈卟啉单胞菌）和革兰氏阳性菌（粪肠球菌和血链球菌）的有效性。评估了冲洗量和暴露时间对HOCl抗菌效果的影响，并完成了耐久性分析。在用三种化疗药物处理后的喷砂处理且被生物膜污染的钛合金圆盘上，进行了活/死染色、形态观察、alamarBlue检测和脂多糖（LPS）检测。结果表明，随着冲洗量增加，HOCl表现出更好的抗菌效果。随着处理时间延长，HOCl实现了更大的抗菌效果。当HOCl开封并与空气接触时，观察到抗菌效果下降。所有冲洗剂均显示出抗菌活性，并杀死了生物膜污染种植体钛合金表面的大部分细菌。此外，与NaOCl和CHX相比，HOCl显著降低了牙龈卟啉单胞菌的LPS浓度。因此，HOCl消毒剂可能对清洁生物膜污染的种植体表面有效。
