Said Mohammed El-Amin, Militello Marcello, Saia Sergio, Settanni Luca, Aleo Aurora, Mammina Caterina, Bombarda Isabelle, Vanloot Pierre, Roussel Christian, Dupuy Nathalie
EA4672 LISA Equipe METICA, Aix-Marseille Université, Case 451, Av. Escadrille Normandie Niémen, FR-13397, Marseille Cedex 20.
Department of Agricultural and Forest Sciences (SAF), Università degli Studi di Palermo, Viale delle Scienze, Ed. 4, IT-90128, Palermo.
Chem Biodivers. 2016 Aug;13(8):1095-102. doi: 10.1002/cbdv.201500510.
Aerial parts of Artemisia arborescens were collected from different sites of the Mediterranean area (southwestern Algeria and southern Italy) and the chemical composition of their essential oil (EO) extracted by hydrodistillation was studied by both gas chromatography (GC) equipped with an enantioselective capillary column and GC/mass spectrometry (GC/MS). The EOs obtained were tested against several Listeria monocytogenes strains. Using GC and GC/MS, 41 compounds were identified, accounting for 96.0 - 98.8% of the total EO. All EOs showed a similar terpene profile, which was rich in chamazulene, β-thujone, and camphor. However, the concentration of such compounds varied among the EOs. A. arborescens EO inhibited up to 83.3% of the L. monocytogenes strains, but the inhibitory spectrum varied among the EOs, with those from Algeria showing a higher inhibition degree than the Italian EOs. Such effect likely depended on the ketone (β-thujone + camphor) content of the EO. The differences in the EO composition support the hypothesis that A. arborescens has at least two different chemotypes: a β-thujone and a chamazulene type. The EO inhibitory spectrum indicates the A. arborescens EO as a valuable option in the control of the food-borne pathogens.
从地中海地区（阿尔及利亚西南部和意大利南部）的不同地点采集了木本蒿的地上部分，采用配备对映选择性毛细管柱的气相色谱（GC）和气相色谱/质谱联用（GC/MS）技术，对经水蒸馏提取的其挥发油（EO）的化学成分进行了研究。所获得的挥发油针对几种单核细胞增生李斯特菌菌株进行了测试。通过GC和GC/MS鉴定出41种化合物，占挥发油总量的96.0%-98.8%。所有挥发油均呈现出相似的萜类成分，富含天蓝烃、β-侧柏酮和樟脑。然而，这些化合物的浓度在不同挥发油之间存在差异。木本蒿挥发油对高达83.3%的单核细胞增生李斯特菌菌株具有抑制作用，但不同挥发油的抑制谱有所不同，来自阿尔及利亚的挥发油显示出比意大利挥发油更高的抑制程度。这种效果可能取决于挥发油中的酮类（β-侧柏酮+樟脑）含量。挥发油成分的差异支持了木本蒿至少有两种不同化学型的假设：β-侧柏酮型和天蓝烃型。挥发油的抑制谱表明木本蒿挥发油是控制食源性病原体的一种有价值的选择。
