Xu Dan, Zhang Bing-Yang, Yang Xiao-Long
Innovative Drug Research Centre, Chongqing University (Huxi Campus), Chongqing, 401331, P. R. China.
College of Pharmaceutical Science, Hebei University, Baoding, 071002, P. R. China.
Chem Biodivers. 2016 Oct;13(10):1422-1425. doi: 10.1002/cbdv.201600114.
A new monoterpene lactone, (1R,4R,5R,8S)-8-hydroxy-4,8-dimethyl-2-oxabicyclo[3.3.1]nonan-3-one (1), along with one related known compound, (2R)-2-[(1R)-4-methylcyclohex-3-en-1-yl]propanoic acid (2), were isolated from the liquid culture of the plant endophytic fungus Pestalotiopsis foedan obtained from the branch of Bruguiera sexangula. The structure and absolute configuration of 1 were determined on the basis of extensive analysis of NMR spectra combined with computational methods via calculation of the optical rotation (OR) and C-NMR. Both compounds exhibited strong antifungal activities against Botrytis cinerea and Phytophthora nicotianae with MIC values of 3.1 and 6.3 μg/ml, respectively, which are comparable to those of the known antifungal drug ketoconazole. Compound 2 also showed modest antifungal activity against Candida albicans with a MIC value of 50 μg/ml.
从采自木榄枝条的植物内生真菌污色拟盘多毛孢（Pestalotiopsis foedan）的液体培养物中分离得到一个新的单萜内酯，即(1R,4R,5R,8S)-8-羟基-4,8-二甲基-2-氧杂双环[3.3.1]壬烷-3-酮（1），以及一个相关已知化合物，即(2R)-2-[(1R)-4-甲基环己-3-烯-1-基]丙酸（2）。通过对核磁共振谱进行广泛分析，并结合计算方法，经旋光（OR）计算和碳核磁共振确定了1的结构和绝对构型。这两种化合物对灰葡萄孢（Botrytis cinerea）和烟草疫霉（Phytophthora nicotianae）均表现出较强的抗真菌活性，其最低抑菌浓度（MIC）值分别为3.1和6.3 μg/ml，与已知抗真菌药物酮康唑相当。化合物2对白色念珠菌（Candida albicans）也表现出一定的抗真菌活性，MIC值为50 μg/ml。
