Guo Jing, Xiang Yang, Peng You-Fan, Huang Hua-Tuo, Lan Yan, Wei Ye-Sheng
Department of Dermatology, Affiliated Hospital of Youjiang Medical University for Nationalities, China.
Youjiang Medical University for Nationalities, China.
Mol Immunol. 2016 Sep;77:1-7. doi: 10.1016/j.molimm.2016.07.001. Epub 2016 Jul 21.
The serum level of IL-33 was upregulated in systemic lupus erythematosus (SLE), which may be used as biomarkers and/or therapeutic targets for SLE. The aim of this study was to investigate the association of four novel polymorphisms of IL-33 with risk of SLE. The study population comprised 540 Chinese individuals, including 257subjects with SLE and 283 healthy controls. The gene polymorphism was measured using Snapshot SNP genotyping assays and confirmed by sequencing. Serum IL-33 (sIL-33) levels were measured by ELISA. The serum SLE levels were significantly higher in the group of patients with SLE than those in the control group (P<0.001). The rs1891385C allele was associated with a significantly increased risk of SLE as compared with the rs1891385 A allele (OR=1.405, 95% CI, 1.052-1.875, P=0.021). The C-T-T-G haplotype was significantly increased the risk of SLE (OR=1.411; 95% CI, 1.021-1.948; P=0.036). IL-33 gene rs1891385 polymorphism was significantly associated with the expression of sIL-33 in the SLE patients. Besides, in our study, we found that the unregulated sIL-33 level was significantly associated with the abnormal changed CRP and ESR. This is the first study reporting the IL-33 gene polymorphisms and SLE, which may help refine the SLE risk profile.
系统性红斑狼疮（SLE）患者血清白细胞介素-33（IL-33）水平上调，其可作为SLE的生物标志物和/或治疗靶点。本研究旨在探讨IL-33四个新的单核苷酸多态性与SLE发病风险的相关性。研究人群包括540名中国个体，其中257例SLE患者和283名健康对照。采用Snapshot SNP基因分型检测法测定基因多态性，并通过测序进行验证。采用酶联免疫吸附测定法（ELISA）检测血清IL-33（sIL-33）水平。SLE患者组血清SLE水平显著高于对照组（P<0.001）。与rs1891385 A等位基因相比，rs1891385 C等位基因与SLE发病风险显著增加相关（比值比[OR]=1.405，95%可信区间[CI]为1.0521.875，P=0.021）。C-T-T-G单倍型显著增加SLE发病风险（OR=1.411；95%CI为1.0211.948；P=0.036）。IL-33基因rs1891385多态性与SLE患者sIL-33表达显著相关。此外，在本研究中，我们发现sIL-33水平失调与C反应蛋白（CRP）和红细胞沉降率（ESR）异常变化显著相关。这是首次报道IL-33基因多态性与SLE的研究，可能有助于完善SLE风险评估。
