Mueller Stefanie J, Hoernstein Sebastian N W, Reski Ralf
INRES-Chemical Signalling University of Bonn, Friedrich-Ebert-Allee 144, DE-53113 Bonn, Germany.
Plant Biotechnology, Faculty of Biology, University of Freiburg, Schaenzlestr.1, DE-79104 Freiburg, Germany.
Mitochondrion. 2017 Mar;33:38-44. doi: 10.1016/j.mito.2016.07.007. Epub 2016 Jul 19.
Extant basal land plants are routinely used to trace plant evolution and to track strategies for high abiotic stress resistance. Whereas the structure of mitochondrial genomes and RNA editing are already well studied, mitochondrial proteome research is restricted to a few data sets. While the mitochondrial proteome of the model moss Physcomitrella patens is covered to an estimated 15-25% by proteomic evidence to date, the available data have already provided insights into the evolution of metabolic compartmentation, dual targeting and mitochondrial heterogeneity. This review summarizes the current knowledge about the mitochondrial proteome of P. patens, and gives a perspective on its use as a mitochondrial model system. Its amenability to gene editing, metabolic labelling as well as fluorescence microscopy provides a unique platform to study open questions in mitochondrial biology, such as regulation of protein stability, responses to stress and connectivity to other organelles. Future challenges will include improving the proteomic resources for P. patens, and to link protein inventories and modifications as well as evolutionary differences to the functional level.
现存的基部陆地植物经常被用于追溯植物进化以及探寻高抗非生物胁迫的策略。虽然线粒体基因组的结构和RNA编辑已经得到了充分研究，但线粒体蛋白质组研究仅限于少数数据集。尽管截至目前，通过蛋白质组学证据估计模式苔藓小立碗藓的线粒体蛋白质组覆盖率为15%-25%，但现有数据已经为代谢区室化、双重靶向和线粒体异质性的进化提供了见解。本综述总结了关于小立碗藓线粒体蛋白质组的当前知识，并展望了其作为线粒体模型系统的应用前景。它易于进行基因编辑、代谢标记以及荧光显微镜观察，为研究线粒体生物学中的开放性问题提供了独特平台，例如蛋白质稳定性的调节、对胁迫的反应以及与其他细胞器的连接。未来的挑战将包括改善小立碗藓的蛋白质组学资源，以及将蛋白质清单、修饰以及进化差异与功能水平联系起来。
