State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
National Key Laboratory of Bio-membrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Science, Beijing, China.
Food Chem. 2016 Dec 15;213:478-484. doi: 10.1016/j.foodchem.2016.06.116. Epub 2016 Jul 2.
A multiplex lateral flow immunoassay (LFA) is developed for the simultaneous on-site determination of three mycotoxins (aflatoxin B1, zearalenone and ochratoxin A) in corn, rice and peanut. By systematically optimizing the preparation of antibody-gold nanoparticle conjugates, the size of gold nanoparticle and the position of capture antigen, the developed LFA can obtain a visual detection limit of 10μg/kg for aflatoxin B1, 50μg/kg for zearalenone and 15μg/kg for ochratoxin A. For quantitative analysis, the limits of detection were 0.10-0.13μg/kg for aflatoxin B1, 0.42-0.46μg/kg for zearalenone, and 0.19-0.24μg/kg for ochratoxin A, which were far below the regulatory limits set by the European Commission. At the spiked concentrations of 0.5-10.0μg/kg, the mean recoveries of the three mycotoxins ranged from 86.2 to 114.5% with coefficients of variation less than 16.7%. These results demonstrated that the developed immunoassay can be used for routine monitoring of mycotoxin contamination.
建立了一种用于同时现场测定玉米、大米和花生中三种霉菌毒素（黄曲霉毒素 B1、玉米赤霉烯酮和赭曲霉毒素 A）的多重侧向流免疫分析（LFA）。通过系统优化抗体-金纳米颗粒缀合物的制备、金纳米颗粒的大小和捕获抗原的位置，开发的 LFA 可以获得黄曲霉毒素 B1 的目视检测限为 10μg/kg，玉米赤霉烯酮为 50μg/kg，赭曲霉毒素 A 为 15μg/kg。对于定量分析，黄曲霉毒素 B1 的检测限为 0.10-0.13μg/kg，玉米赤霉烯酮为 0.42-0.46μg/kg，赭曲霉毒素 A 为 0.19-0.24μg/kg，均远低于欧盟委员会设定的监管限量。在 0.5-10.0μg/kg 的添加浓度下，三种霉菌毒素的平均回收率在 86.2%至 114.5%之间，变异系数小于 16.7%。这些结果表明，开发的免疫分析可以用于常规监测霉菌毒素污染。
