Chen Ruipeng, Wang Hui, Sun Chaoqun, Zhao Yiguang, He Yue, Nisar Muhammad Shemyal, Wei Wensong, Kang Haiqi, Xie Xiulan, Du Chunmei, Luo Qingyao, Yang Liang, Tang Xiangfang, Xiong Benhai
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
College of Animal Science & Technology, Guangxi University, Nanning, Guangxi, 530004, China.
Talanta. 2023 Jun 1;258:124401. doi: 10.1016/j.talanta.2023.124401. Epub 2023 Feb 28.
Agricultural products are frequently contaminated by mycotoxins. Multiplex, ultrasensitive, and rapid determination of mycotoxins is still a challenging problem, which is of great significance to food safety and public health. Herein, a surface-enhanced Raman scattering (SERS) based lateral flow immunoassay (LFA) for the simultaneous on-site determination of aflatoxin B (AFB) and ochratoxin A (OTA) on the same test line (T line) was developed, in this study. In practice, two kinds of Raman reporters 4-mercaptobenzoic acid (4-MBA), and 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) encoded silica-encapsulated gold nanotags (Au@SiO and Au@SiO) were used as detection markers to identify the two different mycotoxins. Through systematic optimization of the experimental conditions, this biosensor has high sensitivity and multiplexing with the limits of detection (LODs) at 0.24 pg mL for AFB and 0.37 pg mL for OTA. These are far below the regulatory limits set by the European Commission, in which the minimum LODs for AFB and OTA are 2.0 and 3.0 μg kg. In the spiked experiment, the food matrix are corn, rice, and wheat, and the mean recoveries of the two mycotoxins ranged from 91.0% ± 6.3%-104.8% ± 5.6% for AFB and 87.0% ± 4.2%-112.0% ± 3.3% for OTA. These results demonstrate that the developed immunoassay has good stability, selectivity, and reliability, which can be used for routine monitoring of mycotoxin contamination.
农产品经常被霉菌毒素污染。对霉菌毒素进行多重、超灵敏且快速的检测仍然是一个具有挑战性的问题，这对食品安全和公众健康具有重要意义。在本研究中，开发了一种基于表面增强拉曼散射（SERS）的侧向流动免疫分析（LFA）方法，用于在同一条检测线（T线）上同时现场测定黄曲霉毒素B（AFB）和赭曲霉毒素A（OTA）。在实际操作中，两种拉曼报告分子4-巯基苯甲酸（4-MBA）和5,5'-二硫代双（2-硝基苯甲酸）（DTNB）编码的二氧化硅包裹金纳米标签（Au@SiO₂和Au@SiO₂）被用作检测标记物，以识别两种不同的霉菌毒素。通过系统优化实验条件，该生物传感器具有高灵敏度和多重检测能力，AFB的检测限（LOD）为0.24 pg/mL，OTA的检测限为0.37 pg/mL。这些远远低于欧盟委员会设定的监管限值，其中AFB和OTA的最低LOD分别为2.0和3.0 μg/kg。在加标实验中，食品基质为玉米、大米和小麦，两种霉菌毒素的平均回收率对于AFB为91.0%±6.3%-104.8%±5.6%，对于OTA为87.0%±4.2%-112.0%±3.3%。这些结果表明，所开发的免疫分析方法具有良好的稳定性、选择性和可靠性，可以用于霉菌毒素污染的常规监测。
