Viana Marques Daniela A, Santos-Ebinuma Valéria C, Pessoa-Júnior Adalberto, Porto Ana L F, Torres Beatriz Rivas, Converti Attilio
Serra Talhada Campus, University of Pernambuco, Serra Talhada-PE, Brazil, Serra Talhada, PE, Brazil.
Dept. of Bioprocess and Biotechnology, School of Pharmaceutical Sciences, UNESP-Univ. Estadual Paulista, Rodovia Araraquara-Jaú Km 1, Araraquara-SP, 14801-902, Brazil.
Biotechnol Prog. 2016 Nov;32(6):1444-1452. doi: 10.1002/btpr.2333. Epub 2016 Aug 11.
In this work, the effects of agitation and aeration rates on aqueous two-phase system (ATPS)-based extractive fermentation of clavulanic acid (CA) by Streptomyces variabilis DAUFPE 3060 were investigated through a 2 full factorial design, where oxygen transfer rate (OTR) and oxygen uptake rate (OUR) were selected as the responses. Aeration rates significantly influenced cell growth, OUR, and CA yield, while OTR was practically the same in all the runs. Under the intermediate agitation (950 rpm) and aeration conditions (3.5 vvm) of the central point runs, it was achieved OTR of 1.617 ± 0.049 mmol L h , OUR of 0.132 ± 0.030 mmol L h , maximum CA production of 434 ± 4 mg L , oxygen mass transfer coefficient of 33.40 ± 2.01 s , partition coefficient of 66.5 ± 1.5, CA yield in the top and bottom phases of 75% ± 2% and 19% ± 1%, respectively, mass balance of 95% ± 4% and purification factor of 3.8 ± 0.1. These results not only confirmed the paramount role of O supply, broth composition and operational conditions in CA ATPS-extractive fermentation, but also demonstrated the possibility of effectively using this technology as a cheap tool to simultaneously produce and recover CA. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1444-1452, 2016.
在本研究中，通过二水平全因子设计，研究了搅拌速率和通气速率对可变链霉菌DAUFPE 3060基于双水相体系（ATPS）的克拉维酸（CA）萃取发酵的影响，其中选择氧传递速率（OTR）和氧摄取速率（OUR）作为响应指标。通气速率显著影响细胞生长、OUR和CA产量，而在所有实验中OTR实际上是相同的。在中心点实验的中等搅拌（950 rpm）和通气条件（3.5 vvm）下，实现了OTR为1.617±0.049 mmol·L⁻¹·h⁻¹，OUR为0.132±0.030 mmol·L⁻¹·h⁻¹，最大CA产量为434±4 mg·L⁻¹，氧传质系数为33.40±2.01 s⁻¹，分配系数为66.5±1.5，CA在上相和下相的产率分别为75%±2%和19%±1%，质量平衡为95%±4%，纯化因子为3.8±0.1。这些结果不仅证实了供氧、发酵液组成和操作条件在CA双水相萃取发酵中的至关重要作用，还证明了有效利用该技术作为一种廉价工具同时生产和回收CA的可能性。© 2016美国化学工程师学会生物技术进展，32:1444 - 1452，2016。
