Research and Development Center of Biorational Pesticides, Northwest Agriculture & Forestry University, Yangling 712100, Shaanxi, China.
Shaanxi Research Center of Biopesticide Engineering & Technology, Yangling 712100, Shaanxi, China.
Molecules. 2018 Jan 11;23(1):125. doi: 10.3390/molecules23010125.
The effects of temperature, agitation and aeration on glycoprotein GP-1 production by ZX01 in bench-scale fermentors were systematically investigated. The maximum final GP-1 production was achieved at an agitation speed of 200 rpm, aeration rate of 2.0 vvm and temperature of 30 °C. By using a dynamic gassing out method, the effects of agitation and aeration on volumetric oxygen transfer coefficient (a) were also studied. The values of volumetric oxygen transfer coefficient in the logarithmic phase increased with increase of agitation speed (from 14.53 to 32.82 h) and aeration rate (from 13.21 to 22.43 h). In addition, a successful scale-up from bench-scale to pilot-scale was performed based on volumetric oxygen transfer coefficient, resulting in final GP-1 production of 3.92, 4.03, 3.82 and 4.20 mg/L in 5 L, 15 L, 70 L and 500 L fermentors, respectively. These results indicated that constant volumetric oxygen transfer coefficient was appropriate for the scale-up of batch fermentation of glycoprotein GP-1 by ZX01, and this scale-up strategy successfully achieved 100-fold scale-up from bench-scale to pilot-scale fermentor.
在中试发酵罐中，系统研究了温度、搅拌和通气对 ZX01 生产糖蛋白 GP-1 的影响。在搅拌速度为 200 rpm、通气速率为 2.0 vvm 和温度为 30°C 的条件下，最终达到了最大 GP-1 产量。通过使用动态鼓泡出气法，还研究了搅拌和通气对体积氧传递系数（a）的影响。对数期的体积氧传递系数随着搅拌速度（从 14.53 增加到 32.82 h）和通气速率（从 13.21 增加到 22.43 h）的增加而增加。此外，基于体积氧传递系数，成功地从中试规模放大到生产规模，在 5 L、15 L、70 L 和 500 L 发酵罐中，最终 GP-1 的产量分别为 3.92、4.03、3.82 和 4.20 mg/L。这些结果表明，对于 ZX01 生产糖蛋白 GP-1 的分批发酵，恒定的体积氧传递系数适用于放大，并且这种放大策略成功地实现了从中试发酵罐到生产规模发酵罐的 100 倍放大。
