Zhang Chao, Sun Lei, Chen Rong, Su Jing-Hua, Zhang Hua-Feng, Gu Bing-Ren, Xing Yi-Wen, Xue Man
Suzhou Institute for Food and Drug Control, Suzhou, Jiangsu, China.
National Institutes for Food and Drug Control, Beijing, China.
J Sep Sci. 2016 Sep;39(18):3536-43. doi: 10.1002/jssc.201600356. Epub 2016 Aug 22.
Quantitative analysis for biological single molecules in Fritillariae Thunbergii Bulbus and identification method by chemometrics was established by using high-performance liquid chromatography. Amino acids, nucleosides, and nucleobases were quantitatively analyzed, and 11 peaks were selected for species identification. A Common pattern was established for chemometrics, and then similarity analysis, principal component analysis, and hierarchical cluster analysis heatmap were applied, and the results indicated that species were ideally identified from the adulterants as Fritillariae Cirrhosae Bulbus. This evaluation method was valuable for further quality control to select the characteristic components.
采用高效液相色谱法建立了浙贝母中生物单分子的定量分析及化学计量学鉴别方法。对氨基酸、核苷和核碱基进行了定量分析，并选择了11个峰进行物种鉴别。建立了化学计量学的通用模式，然后进行相似度分析、主成分分析和层次聚类分析热图，结果表明该物种能够理想地从混淆品川贝母中鉴别出来。该评价方法对于进一步筛选特征成分进行质量控制具有重要价值。
