Liu Lei, Lan Gongbin, Peng Longkai, Xie Xubiao, Peng Fenghua, Yu Shaojie, Wang Yu, Tang Xiaotian
a Department of Urological Organ Transplantation , The Second Xiangya Hospital, Central South University , Changsha , China.
Ren Fail. 2016 Sep;38(8):1199-205. doi: 10.1080/0886022X.2016.1208517. Epub 2016 Jul 25.
NDUFA4L2 is overexpressed in VHL-deficient cell lines and neuroblastoma. The clinical significance of NDUFA4L2 in clear cell renal cell carcinoma (ccRCC) has not been well studied. Therefore, we evaluated the prognostic value of NDUFA4L2 in ccRCC patients.
In our study, NDUFA4L2 expression in 86 cases of ccRCC and adjacent normal tissues was monitored by immunohistochemistry, semi-quantitative RT-PCR, and Western blot analyses. The relationship between NDUFA4L2 expression and the clinical features of ccRCC was assessed.
The results showed that NDUFA4L2 protein expression was found to be higher in ccRCC tissues 81.4% (70/86) than in normal tissues 26.7% (23/86) (p = 0.021). The average level of NDUFA4L2 mRNA expression was found to be 122.23 ± 6.018 and 21.34 ± 1.036 in ccRCC tissue and adjacent normal tissue (p < 0.001). NDUFA4L2 expression levels were correlated with some clinical features of ccRCC. Multivariate analysis showed NDUFA4L2 expression was an independent prognostic factor for ccRCC patients.
Our study has provided the significant clinical relevance of NDUFA4L2 in ccRCC and suggested that ccRCC patients with NDUFA4L2 overexpression may be suitable as a potential therapeutic target for ccRCC patients.
NDUFA4L2在VHL缺陷细胞系和神经母细胞瘤中过表达。其在透明细胞肾细胞癌（ccRCC）中的临床意义尚未得到充分研究。因此，我们评估了NDUFA4L2在ccRCC患者中的预后价值。
在我们的研究中，通过免疫组织化学、半定量逆转录-聚合酶链反应（RT-PCR）和蛋白质印迹分析监测了86例ccRCC及癌旁正常组织中NDUFA4L2的表达。评估了NDUFA4L2表达与ccRCC临床特征之间的关系。
结果显示，NDUFA4L2蛋白表达在81.4%（70/86）的ccRCC组织中高于26.7%（23/86）的正常组织（p = 0.021）。NDUFA4L2 mRNA表达的平均水平在ccRCC组织和癌旁正常组织中分别为122.23 ± 6.018和21.34 ± 1.036（p < 0.001）。NDUFA4L2表达水平与ccRCC的一些临床特征相关。多因素分析显示，NDUFA4L2表达是ccRCC患者的独立预后因素。
我们的研究揭示了NDUFA4L2在ccRCC中的重要临床意义，并表明NDUFA4L2过表达的ccRCC患者可能适合作为ccRCC患者潜在的治疗靶点。
