Apanovich Natalya, Apanovich Pavel, Mansorunov Danzan, Kuzevanova Anna, Matveev Vsevolod, Karpukhin Alexander
Laboratory of Molecular Genetics of Complex Inherited Diseases, Research Centre for Medical Genetics, Moscow, Russia.
Department of Oncourology, Federal State Budgetary Institution "N.N. Blokhin National Medical Research Center of Oncology" of the Ministry of Health of the Russian Federation, Moscow, Russia.
Front Oncol. 2021 May 11;11:615787. doi: 10.3389/fonc.2021.615787. eCollection 2021.
We aimed to identify and investigate genes that are essential for the development of clear cell renal cell carcinoma (ccRCC) and sought to shed light on the mechanisms of its progression and create prognostic markers for the disease. We used real-time PCR to study the expression of 20 genes that were preliminarily selected based on their differential expression in ccRCC, in 68 paired tumor/normal samples. Upon ccRCC progression, seven genes that showed an initial increase in expression showed decreased expression. The genes whose expression levels did not significantly change during progression were associated mainly with metabolic and inflammatory processes. The first group included , , , , , , and , whose expression levels were coordinately decreased during tumor progression. This expression coordination and gene function is related to the needs of tumor development at different stages. Specifically, the high correlation coefficient of and expression may indicate the importance of the coordinated regulation of glycolysis and mitochondrial metabolism. A panel of , , , and enabled the prediction of survival for more than 3.5 years in patients with ccRCC, with a probability close to 90%. Therefore, a coordinated change in the expression of a gene group during ccRCC progression was detected, and a new panel of markers for individual survival prognosis was identified.
我们旨在识别和研究对透明细胞肾细胞癌（ccRCC）发展至关重要的基因，并试图阐明其进展机制，为该疾病创建预后标志物。我们使用实时PCR研究了基于在ccRCC中差异表达而初步选择的20个基因在68对肿瘤/正常样本中的表达。在ccRCC进展过程中，最初表达增加的7个基因表达下降。在进展过程中表达水平无显著变化的基因主要与代谢和炎症过程相关。第一组包括 、 、 、 、 、 和 ，其表达水平在肿瘤进展过程中协同下降。这种表达协同作用和基因功能与肿瘤在不同阶段发展的需求相关。具体而言， 和 表达的高相关系数可能表明糖酵解和线粒体代谢协同调节的重要性。一组 、 、 和 能够预测ccRCC患者超过3.5年的生存期，概率接近90%。因此，检测到ccRCC进展过程中基因组成的表达协同变化，并鉴定出用于个体生存预后的新标志物组。
