一个新基因OsAHL1可提高水稻的避旱性和耐旱性。
A novel gene OsAHL1 improves both drought avoidance and drought tolerance in rice.
作者信息
Zhou Liguo, Liu Zaochang, Liu Yunhua, Kong Deyan, Li Tianfei, Yu Shunwu, Mei Hanwei, Xu Xiaoyan, Liu Hongyan, Chen Liang, Luo Lijun
机构信息
Shanghai Agrobiological Gene Center, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
National Key Laboratory of Crop Genetic Improvement, College of Plant Science, Huazhong Agricultural University, Wuhan 430070, China.
出版信息
Sci Rep. 2016 Jul 25;6:30264. doi: 10.1038/srep30264.
A novel gene, OsAHL1, containing an AT-hook motif and a PPC domain was identified through genome-wide profiling and analysis of mRNAs by comparing the microarray of drought-challenged versus normally watered rice. The results indicated OsAHL1 has both drought avoidance and drought tolerance that could greatly improve drought resistance of the rice plant. Overexpression of OsAHL1 enhanced multiple stress tolerances in rice plants during both seedling and panicle development stages. Functional studies revealed that OsAHL1 regulates root development under drought condition to enhance drought avoidance, participates in oxidative stress response and also regulates the content of chlorophyll in rice leaves. OsAHL1 specifically binds to the A/T rich sequence region of promoters or introns, and hence directly regulates the expression of many stress related downstream genes.
通过对干旱胁迫水稻与正常浇水水稻的微阵列进行比较，在全基因组范围内对mRNA进行分析和谱分析，鉴定出一个含有AT钩基序和PPC结构域的新基因OsAHL1。结果表明，OsAHL1具有避旱和耐旱能力，可大大提高水稻植株的抗旱性。OsAHL1的过表达增强了水稻植株在幼苗期和穗发育阶段的多种胁迫耐受性。功能研究表明，OsAHL1在干旱条件下调节根系发育以增强避旱能力，参与氧化应激反应，并调节水稻叶片中的叶绿素含量。OsAHL1特异性结合启动子或内含子的富含A/T的序列区域，从而直接调节许多胁迫相关下游基因的表达。
Zotero 插件