Bodero Maria, Francisco Abisambra Jose
Sanders-Brown Center on Aging and Department of Physiology, University of Kentucky, Lexington, USA.
Bio Protoc. 2014 Feb 5;4(3). doi: 10.21769/bioprotoc.1038.
This protocol details the extraction of microsomes from frozen tissue in order to further examine the protein-protein interactions occurring within the endoplasmic reticulum. This protocol was adapted from Abisambra (2013) with modifications made in order to optimize for subsequent use.
本方案详细介绍了从冷冻组织中提取微粒体的方法，以便进一步研究内质网内发生的蛋白质-蛋白质相互作用。本方案改编自阿比萨布拉（2013年），并进行了修改以优化后续使用。
