采用汞化亲和柱进行样品净化后，通过带氮磷检测的气相色谱法和气相色谱-质谱法对尿液中的抗甲状腺药物进行筛查和确证。

Screening and confirmation of thyreostatics in urine by gas chromatography with nitrogen-phosphorus detection and gas chromatography-mass spectrometry after sample clean-up with a mercurated affinity column.

作者信息

Schilt R, Weseman J M, Hooijerink H, Korbee H J, Traag W A, van Steenbergen M J, Haasnoot W

机构信息

State Institute for Quality Control of Agricultural Products (RIKILT), Wageningen, The Netherlands.

出版信息

J Chromatogr. 1989 Apr 7;489(1):127-37. doi: 10.1016/s0378-4347(00)82890-x.

DOI:10.1016/s0378-4347(00)82890-x

PMID:2745644

Abstract

Methods are described for the screening and confirmation of residues of the thyreostatics thiouracil, methylthiouracil and propylthiouracil in urine samples of cattle at levels down to 25 micrograms/l. After a selective preconcentration of the thiol-containing thyreostatics on a mercurated affinity column, the analytes are derivatized by extractive alkylation and analysed by gas chromatography with nitrogen-phosphorus or mass spectrometric detection.

摘要

本文描述了用于筛查和确证牛尿液样本中抗甲状腺药硫脲嘧啶、甲基硫脲嘧啶和丙基硫脲嘧啶残留的方法，检测下限可达25微克/升。在汞化亲和柱上对含硫醇的抗甲状腺药进行选择性预富集后，通过萃取烷基化对分析物进行衍生化，并采用氮磷检测或质谱检测的气相色谱法进行分析。