Jansen E H, Buskens C A, van den Berg R H
Laboratory for Clinical Chemistry, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
J Chromatogr. 1989 Apr 7;489(1):245-53. doi: 10.1016/s0378-4347(00)82902-3.
Chemiluminescent immunoassays performed in microtitre plates can be detected with a sensitive instant photoplate. An apparatus was constructed for this purpose making use of whole polystyrene microtitre plates plates possible. One of the advantages of immunoassays using enzyme-enhanced chemiluminescence is the possibility for developing homogeneous immunoassays. An example is described for a solid-phase immunoassay for 17 beta-testosterone making use of non-specific energy transfer in combination with photoplate detection. A testosterone-peroxidase conjugate was used as label. Quantification was performed using image-analysis techniques. The assay was also performed with colorimetric substrates for comparison.
在微量滴定板中进行的化学发光免疫测定可以用灵敏的即时感光板进行检测。为此构建了一种装置，使得使用整个聚苯乙烯微量滴定板成为可能。使用酶增强化学发光的免疫测定的优点之一是开发均相免疫测定的可能性。描述了一个利用非特异性能量转移结合感光板检测进行17β-睾酮固相免疫测定的实例。使用睾酮-过氧化物酶结合物作为标记。使用图像分析技术进行定量。还使用比色底物进行了该测定以作比较。
