Uribe Juan E, Colgan Don, Castro Lyda R, Kano Yasunori, Zardoya Rafael
Museo Nacional de Ciencias Naturales (MNCN-CSIC), José Gutiérrez Abascal 2, 28006 Madrid, Spain.
AMRI, The Australian Museum, 1 William Street, Sydney 2010, Australia.
Mol Phylogenet Evol. 2016 Nov;104:21-31. doi: 10.1016/j.ympev.2016.07.021. Epub 2016 Jul 22.
Despite the extraordinary morphological and ecological diversity of Neritimorpha, few studies have focused on the phylogenetic relationships of this lineage of gastropods, which includes four extant superfamilies: Neritopsoidea, Hydrocenoidea, Helicinoidea, and Neritoidea. Here, the nucleotide sequences of the complete mitochondrial genomes of Georissa bangueyensis (Hydrocenoidea), Neritina usnea (Neritoidea), and Pleuropoma jana (Helicinoidea) and the nearly complete mt genomes of Titiscania sp. (Neritopsoidea) and Theodoxus fluviatilis (Neritoidea) were determined. Phylogenetic reconstructions using probabilistic methods were based on mitochondrial (13 protein coding genes and two ribosomal rRNA genes), nuclear (partial 28S rRNA, 18S rRNA, actin, and histone H3 genes) and combined sequence data sets. All phylogenetic analyses except one converged on a single, highly supported tree in which Neritopsoidea was recovered as the sister group of a clade including Helicinoidea as the sister group of Hydrocenoidea and Neritoidea. This topology agrees with the fossil record and supports at least three independent invasions of land by neritimorph snails. The mitochondrial genomes of Titiscania sp., G. bangueyensis, N. usnea, and T. fluviatilis share the same gene organization previously described for Nerita mt genomes whereas that of P. jana has undergone major rearrangements. We sequenced about half of the mitochondrial genome of another species of Helicinoidea, Viana regina, and confirmed that this species shares the highly derived gene order of P. jana.
尽管 Neritimorpha 具有非凡的形态和生态多样性，但很少有研究关注这种腹足纲动物谱系的系统发育关系，该谱系包括四个现存的超科：Neritopsoidea、Hydrocenoidea、Helicinoidea 和 Neritoidea。在这里，测定了 Georissa bangueyensis（Hydrocenoidea）、Neritina usnea（Neritoidea）和 Pleuropoma jana（Helicinoidea）的完整线粒体基因组的核苷酸序列，以及 Titiscania sp.（Neritopsoidea）和 Theodoxus fluviatilis（Neritoidea）的近乎完整的线粒体基因组序列。使用概率方法进行的系统发育重建基于线粒体（13 个蛋白质编码基因和两个核糖体 rRNA 基因）、核（部分 28S rRNA、18S rRNA、肌动蛋白和组蛋白 H3 基因）以及组合序列数据集。除了一次分析外，所有的系统发育分析都汇聚在一棵单一的、得到高度支持的树上，其中 Neritopsoidea 被恢复为一个分支的姐妹群，该分支包括 Helicinoidea 作为 Hydrocenoidea 和 Neritoidea 的姐妹群。这种拓扑结构与化石记录一致，并支持 Neritimorph 蜗牛至少三次独立入侵陆地。Titiscania sp.、G. bangueyensis、N. usnea 和 T. fluviatilis 的线粒体基因组具有先前描述的 Nerita 线粒体基因组相同的基因组织，而 P. jana 的线粒体基因组则经历了重大重排。我们对另一种 Helicinoidea 物种 Viana regina 的线粒体基因组大约一半进行了测序，并证实该物种与 P. jana 具有高度衍生的基因顺序。
