Cacciapuoti A F, Wegener W S, Morse S A
Infect Immun. 1978 May;20(2):418-20. doi: 10.1128/iai.20.2.418-420.1978.
The relationship between conditions which permit or inhibit cell lysis and those which promote phospholipid hydrolysis in Neisseria gonorrhoeae was investigated. Suspension of exponential-phase gonococci in buffer in the absence of divalent cations resulted in autolysis but not in phosphlipid hydrolysis. The addition of Ca2+ or Mg2+ to the buffer inhibited autolysis and markedly stimulated the hydrolysis of phosphatidylethanolamine. Incubation of cells in buffer at pH 6 inhibited both autolysis and phospholipid hydrolysis.
研究了淋病奈瑟菌中允许或抑制细胞裂解的条件与促进磷脂水解的条件之间的关系。在没有二价阳离子的情况下,将对数生长期的淋球菌悬浮于缓冲液中会导致自溶,但不会导致磷脂水解。向缓冲液中添加Ca2+或Mg2+可抑制自溶,并显著刺激磷脂酰乙醇胺的水解。将细胞在pH 6的缓冲液中孵育可同时抑制自溶和磷脂水解。