KIF11 在具有肠型黏液表型的胃癌中的过表达。

Overexpression of KIF11 in Gastric Cancer with Intestinal Mucin Phenotype.

机构信息

Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan.

出版信息

Pathobiology. 2017;84(1):16-24. doi: 10.1159/000447303. Epub 2016 Jul 27.

DOI:10.1159/000447303

PMID:27459100

Abstract

OBJECTIVE

Gastric cancer (GC) is one of the most common human cancers. A useful method of gastric cancer stem cell (CSC) characterization is spheroid colony formation. Previously, we reported that KIF11 expression is >2-fold in spheroid-body-forming GC cells compared with parental cells. Here, we analyzed the expression and distribution of KIF11 in human GC by immunohistochemistry.

METHODS

Expression of KIF11 in 165 GC cases was determined using immunohistochemistry. For mucin phenotypic expression analysis of GC, immunostaining of MUC5AC, MUC6, MUC2 and CD10 was evaluated. RNA interference was used to inhibit KIF11 expression in GC cell lines.

RESULTS

In total, 119 of 165 GC cases (72%) were positive for KIF11. Expression of KIF11 was not associated with any clinicopathologic characteristics; however, it was observed frequently in GC exhibiting an intestinal phenotype. Both the number and size of spheres formed by MKN-74 cells were significantly reduced following transfection of KIF11-targeting siRNA compared with negative-control siRNA. Furthermore, levels of phosphorylated Erk1/2 were lower in KIF11 siRNA-transfected cells than with negative-control siRNA-transfected cells.

CONCLUSION

These results indicate that KIF11 is involved in intestinal mucin phenotype GC.

摘要

目的

胃癌（GC）是最常见的人类癌症之一。一种鉴定胃癌干细胞（CSC）的有效方法是球体集落形成。先前，我们报道了与亲本细胞相比，球体体形成 GC 细胞中的 KIF11 表达增加了 2 倍以上。在这里，我们通过免疫组织化学分析了 KIF11 在人类 GC 中的表达和分布。

方法

用免疫组织化学检测 165 例 GC 病例中 KIF11 的表达。为了分析 GC 的粘蛋白表型表达，评估了 MUC5AC、MUC6、MUC2 和 CD10 的免疫染色。用 RNA 干扰抑制 GC 细胞系中 KIF11 的表达。

结果

总共，在 165 例 GC 病例中，有 119 例（72%）KIF11 阳性。KIF11 的表达与任何临床病理特征无关；然而，在具有肠表型的 GC 中观察到其表达频率较高。与阴性对照 siRNA 转染相比，MKN-74 细胞形成的球体数量和大小在用 KIF11 靶向 siRNA 转染后均显著减少。此外，在 KIF11 siRNA 转染的细胞中磷酸化 Erk1/2 的水平低于阴性对照 siRNA 转染的细胞。