Smidak Roman, Aradska Jana, Kirchberger Stefanie, Distel Martin, Sialana Fernando J, Wackerlig Judith, Mechtcheriakova Diana, Lubec Gert
Department of Pediatrics, Medical University of Vienna, Vienna, Austria.
Children's Cancer Research Institute, St. Anna Kinderkrebsforschung, Innovative Cancer Models, Vienna, Austria.
Proteomics Clin Appl. 2016 Dec;10(12):1264-1268. doi: 10.1002/prca.201600081. Epub 2016 Aug 12.
Zebrafish (Danio rerio) is a well-established model organism in developmental biology and disease modeling. In recent years, an increasing amount of studies used zebrafish to analyze the genetic changes underlying various neurological disorders. The brain plasma membrane proteome represents the major subsets of signaling proteins and promising drug targets, but is often understudied due to traditional experimental difficulties including problems with solubility, detergent removal, or low abundance. Here, we report a comprehensive dataset of the proteins identified in the enriched plasma membrane of the zebrafish brain by applying sequential trypsin/chymotrypsin digestion with multidimensional LC-MS/MS. A total number of 97 017 peptide groups corresponding to 9201 proteins were identified. These were annotated in various molecular functions or neurological disorders. The dataset of the current study provides a useful data source for further utilizing zebrafish in basic and clinical neuroscience.
斑马鱼（Danio rerio）是发育生物学和疾病建模中一种成熟的模式生物。近年来，越来越多的研究使用斑马鱼来分析各种神经系统疾病背后的基因变化。脑质膜蛋白质组代表了信号蛋白的主要子集和有前景的药物靶点，但由于传统实验困难，包括溶解性问题、去污剂去除问题或丰度低等，常常未得到充分研究。在此，我们报告了通过应用胰蛋白酶/糜蛋白酶顺序消化结合多维液相色谱-串联质谱法在斑马鱼脑富集质膜中鉴定出的蛋白质的综合数据集。共鉴定出9201种蛋白质对应的97017个肽组。这些肽组在各种分子功能或神经系统疾病中得到注释。本研究的数据集为在基础和临床神经科学中进一步利用斑马鱼提供了有用的数据源。
