Wiranowska M, Wilson T C, Thompson K, Prockop L D
Department of Neurology, College of Medicine, University of South Florida, Tampa 33612.
J Interferon Res. 1989 Jun;9(3):353-62. doi: 10.1089/jir.1989.9.353.
Systemically administered interferon (IFN) is not readily detected in the central nervous system (CNS) due to the presence of the blood-brain barrier (BBB). A method of osmotic BBB alteration in a mouse model was established in this laboratory. IFN's entry into the normal and osmotically altered brain after its intracarotid injection was investigated. Significant IFN levels (100-1,000 units) in the brain can be achieved by this method. The highest IFN activity was found in the brain hemisphere ipsilateral to the injection site within 20 min to 1 h after injection. IFN activity in the brain was detectable up to 4 h. Animals injected in this manner with murine IFN-alpha/beta (MuIFN-alpha/beta) and observed for a 6-month time interval showed no signs of neurological dysfunctions and resumed their normal activities. The therapeutic value of this method will be tested in a murine model of malignant glioma.
由于血脑屏障（BBB）的存在，全身给药的干扰素（IFN）在中枢神经系统（CNS）中不易被检测到。本实验室建立了一种在小鼠模型中改变血脑屏障渗透压的方法。研究了颈动脉注射IFN后其进入正常和渗透压改变的大脑的情况。通过这种方法可以在大脑中达到显著的IFN水平（100 - 1000单位）。注射后20分钟至1小时内，在注射部位同侧的脑半球中发现最高的IFN活性。大脑中的IFN活性在4小时内均可检测到。以这种方式注射鼠干扰素α/β（MuIFN-α/β）并观察6个月时间间隔的动物未表现出神经功能障碍的迹象，并恢复了正常活动。该方法的治疗价值将在恶性胶质瘤的小鼠模型中进行测试。
