Wiranowska M, Wilson T C, Bencze K S, Prockop L D
Department of Neurology, College of Medicine, University of South Florida, Tampa.
J Neurosci Methods. 1988 Dec;26(2):105-9. doi: 10.1016/0165-0270(88)90158-6.
This article describes a C57BL/6 mouse model for the investigation of blood-brain barrier (BBB) alteration. Osmotic modification of BBB was achieved by infusion of 1.6 M arabinose solution into the internal carotid artery with or without occlusion of the external carotid artery. BBB alteration was measured by infusing 2% Evans blue dye. Only 1.6 M arabinose-treated animals but not 0.9% NaCl controls displayed prominent ipsilateral staining of frontal and temporal lobes. Light blue staining occurred in animals sacrificed within 10 min after injection. Prominent staining occurred in animals sacrificed 1-6 hours later. Identically treated animals were maintained for up to 6 months without signs of systemic or neurological dysfunction. This model may permit study of the effects of biological response modifiers (BRMs) upon the central nervous system (CNS) in healthy and diseased mice.
本文描述了一种用于研究血脑屏障（BBB）改变的C57BL/6小鼠模型。通过向颈内动脉注入1.6M阿拉伯糖溶液，无论是否结扎颈外动脉，均可实现血脑屏障的渗透性改变。通过注入2%伊文思蓝染料来测量血脑屏障的改变。仅1.6M阿拉伯糖处理的动物而非0.9%氯化钠对照组显示出额叶和颞叶明显的同侧染色。注射后10分钟内处死的动物出现浅蓝色染色。1 - 6小时后处死的动物出现明显染色。经相同处理的动物可维持长达6个月，无全身或神经功能障碍迹象。该模型可用于研究生物反应调节剂（BRM）对健康和患病小鼠中枢神经系统（CNS）的影响。
