Ananworanich Jintanat, Chomont Nicolas, Eller Leigh Ann, Kroon Eugene, Tovanabutra Sodsai, Bose Meera, Nau Martin, Fletcher James L K, Tipsuk Somporn, Vandergeeten Claire, O'Connell Robert J, Pinyakorn Suteeraporn, Michael Nelson, Phanuphak Nittaya, Robb Merlin L
U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA; SEARCH, The Thai Red Cross AIDS Research Centre, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
CRCHUM and Department of microbiology, infectiology and immunology, Université de Montréal, Montreal, Canada; The Vaccine and Gene Therapy Institute-Florida, Port St. Lucie, Florida, USA.
EBioMedicine. 2016 Sep;11:68-72. doi: 10.1016/j.ebiom.2016.07.024. Epub 2016 Jul 20.
HIV DNA is a marker of HIV persistence that predicts HIV progression and remission, but its kinetics in early acute HIV infection (AHI) is poorly understood. We longitudinally measured the frequency of peripheral blood mononuclear cells harboring total and integrated HIV DNA in 19 untreated and 71 treated AHI participants, for whom 50 were in the earliest Fiebig I/II (HIV IgM-) stage, that is ≤2weeks from infection. Without antiretroviral therapy (ART), HIV DNA peaked at 2weeks after enrollment, reaching a set-point 2weeks later with little change thereafter. There was a marked divergence of HIV DNA values between the untreated and treated groups that occurred within the first 2weeks of ART and increased with time. ART reduced total HIV DNA levels by 20-fold after 2weeks and 316-fold after 3years. Therefore, very early ART offers the opportunity to significantly reduce the frequency of cells harboring HIV DNA.
HIV DNA是HIV持续存在的一个标志物，可预测HIV的进展和缓解情况，但其在急性HIV感染早期（AHI）的动力学变化仍知之甚少。我们纵向测量了19名未经治疗和71名接受治疗的AHI参与者外周血单个核细胞中携带总HIV DNA和整合HIV DNA的频率，其中50人处于最早的Fiebig I/II期（HIV IgM阴性），即感染后≤2周。在未进行抗逆转录病毒治疗（ART）的情况下，HIV DNA在入组后2周达到峰值，2周后达到稳定水平，此后变化不大。在ART治疗的前2周内，未治疗组和治疗组的HIV DNA值出现明显差异，并随时间增加。ART治疗2周后使总HIV DNA水平降低了20倍，3年后降低了316倍。因此，极早期ART治疗提供了显著降低携带HIV DNA细胞频率的机会。
