Miyauchi F, Inoguchi H, Takasaki A, Tamura H, Kato H, Torigoe T
Department of Obstetrics and Gynecology, Yamaguchi University School of Medicine.
Nihon Sanka Fujinka Gakkai Zasshi. 1989 Apr;41(4):383-9.
Luteal cells from the corpora lutea of rats on day 15 of pregnancy were suspended in DMEM-F12 and progesterone accumulation was measured by radioimmunoassay. Progesterone accumulation increased steadily over eight hours and then gradually decreased. Progesterone accumulation was seen to increase with the addition of BSA and 25-hydroxycholesterol. At doses of 12 ng/ml and 600 ng/ml hCG, progesterone accumulation increased, but was seen to decrease at a dose of 30 micrograms/ml hCG. However, the addition of cyclohexamide alone had no effect on progesterone accumulation, but the simultaneous addition of cyclohexamide and hCG blocked the effects of hCG in a dose related manner. These data indicate that progesterone production by rat luteal cells depends on cholesterol availability. The results reveal that hCG stimulates progesterone production by a process requiring new protein synthesis.
妊娠第15天大鼠黄体的黄体细胞悬浮于DMEM - F12中，通过放射免疫测定法测量孕酮积累量。孕酮积累量在8小时内稳步增加，然后逐渐下降。观察到添加牛血清白蛋白（BSA）和25 - 羟基胆固醇后孕酮积累量增加。当人绒毛膜促性腺激素（hCG）剂量为12 ng/ml和600 ng/ml时，孕酮积累量增加，但当hCG剂量为30微克/毫升时观察到孕酮积累量下降。然而，单独添加环己酰胺对孕酮积累量没有影响，但同时添加环己酰胺和hCG会以剂量相关的方式阻断hCG的作用。这些数据表明大鼠黄体细胞产生孕酮取决于胆固醇的可利用性。结果显示，hCG通过需要新蛋白质合成的过程刺激孕酮的产生。
