巨噬细胞系中人肿瘤坏死因子α/消瘦素启动子区域的遗传分析。
Genetic analysis of the human tumor necrosis factor alpha/cachectin promoter region in a macrophage cell line.
作者信息
Economou J S, Rhoades K, Essner R, McBride W H, Gasson J C, Morton D L
机构信息
Division of Surgical Oncology, John Wayne Cancer Clinic, Armand Hammer Laboratories, Los Angeles, California.
出版信息
J Exp Med. 1989 Jul 1;170(1):321-6. doi: 10.1084/jem.170.1.321.
Abstract
The 615-bp 5' flanking region of the human TNF-alpha/cachectin gene was isolated and ligated to the luciferase reporter gene. In addition, a series of truncated promoter constructs was generated by exonuclease III digestion. The promoter activity of these constructs was studied in a transient transfection system using the TNF-alpha-producing U937 cell line. Full-length and truncated TNF promoter constructions extending from -615 to -95 bp relative to the transcription start site (TSS) could be induced by phorbol esters. A construct truncated to within 36 bp of the TSS (and within 11 bp of the TATAA box) was inactive. Therefore, the phorbol ester responsive is localized in the TNF/cachectin promoter to a relatively short region proximal to the TATAA box.
摘要
分离出人类肿瘤坏死因子-α/恶病质素基因615 bp的5'侧翼区域，并将其连接到荧光素酶报告基因上。此外，通过核酸外切酶III消化产生了一系列截短的启动子构建体。使用产生肿瘤坏死因子-α的U937细胞系，在瞬时转染系统中研究了这些构建体的启动子活性。相对于转录起始位点（TSS），从-615至-95 bp延伸的全长和截短的肿瘤坏死因子启动子构建体可被佛波酯诱导。截短至TSS的36 bp内（以及TATAA框的11 bp内）的构建体无活性。因此，佛波酯反应性定位于肿瘤坏死因子/恶病质素启动子中靠近TATAA框的一个相对较短的区域。
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