Begley C G, Aplan P D, Davey M P, de Villartay J P, Cohen D I, Waldmann T A, Kirsch I R
Navy Medical Oncology, National Cancer Institute, Bethesda, Maryland 20892.
J Exp Med. 1989 Jul 1;170(1):339-42. doi: 10.1084/jem.170.1.339.
It has been hypothesized that a rearrangement between the delta recombining element (delta Rec) and a pseudo J alpha gene serves to delete the TCR-delta locus before rearrangement of the TCR-alpha genes. We have now sequenced a direct, site-specific rearrangement between the delta Rec element and a pseudo J alpha gene in a human leukemic stem-cell line. Putative "N-sequence" addition was noted at the site of recombination, suggesting that this event occurred at a time when the enzyme(s) involved in N-region addition were active in this cell. This provides support for the view that deletion of the TCR-delta locus is required before rearrangement of the TCR-alpha chain genes.
据推测，δ重组元件（δRec）与假Jα基因之间的重排作用是在TCR-α基因重排之前删除TCR-δ基因座。我们现已对人类白血病干细胞系中δRec元件与假Jα基因之间的直接、位点特异性重排进行了测序。在重组位点发现了推定的“N序列”添加，这表明该事件发生在参与N区域添加的酶在该细胞中具有活性的时期。这为TCR-α链基因重排之前需要删除TCR-δ基因座这一观点提供了支持。
